1.5.1.41	construction of a bacterial strain that overproduces the enzyme approximately 100fold	
1.5.1.41	expressed of N-terminally His-tagged HpaCSt protein in Escherichia coli	
1.5.1.41	gene frd1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant overexpression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)