1.6.5.9 expressed in Escherichia coli BL21(DE3) pLysS cells 1.6.5.9 expressed in Escherichia coli BL21(DE3)pLysS cells 1.6.5.9 expressed in Escherichia coli membranes 1.6.5.9 gene Ndi1, inducible NDI1 enzyme expression in Drosophila melanogaster mitochondria of different cells and tissues. Expression of Ndi1 in fly mitochondria leads to an increase in NADH-ubiquinone oxidoreductase activity, oxygen consumption and ATP levels. In addition, exogenous Ndi1 expression results in increased CO2 production in living flies. Targeted expression of Ndi1 in fly neurons significantly increases lifespan without compromising fertility or physical activity 1.6.5.9 recombinant expression of His-tagged NDH-2