2.3.1.84 - 487564, 736589 2.3.1.84 a pPTB construct is used, expression experiments are performed in Escherichia coli cells 702712 2.3.1.84 cloned from the VIN13 commercial wine yeast strain, and placed under the constitutive control of the PGK1 regulatory sequences to generate plasmids pATF2-s. Overexpression of ATF2 in vine yeast increased the concentrations of ethyl acetate and isoamyl acetate to a lesser degree than overexpression of ATF1 677273 2.3.1.84 cloned from the VIN13 commercial wine yeast strain, and placed under the constitutive control of the PGK1 regulatory sequences. Overexpression of ATF1 in vine yeast significantly increased the concentrations of ethyl acetate, isoamyl acetate, 2-phenylethyl acetate and ethyl caproate 677273 2.3.1.84 cloning of ATF1 and ATF2 genes encoding alcohol acetyltransferases 487565 2.3.1.84 cloning of ATF1 gene encoding alcohol acetyltransferase, expression in Saccharomyces cerevisiae 487560 2.3.1.84 cloning of Lg-ATF1 gene encoding alcohol acetyltransferase, expression in Saccharomyces cerevisiae 487560 2.3.1.84 cloning of the ATF1 gene encoding alcohol acetyltransferase 1 from wine yeast 487566 2.3.1.84 complete coding region of PhCFAT cDNA subcloned into expression vector pET-28a and expressed in Escherichia coli 723419 2.3.1.84 expression in Escherichia coli 720138, 735555 2.3.1.84 expression in Escherichia coli and in Clostridium acetobutylicum, alcohol acetyltransferase AFT2 662577 2.3.1.84 expression in Escherichia coli, alcohol acetyltransferase AFT1 662577 2.3.1.84 expression in Saccharomyces cerevisiae 719627, 758549 2.3.1.84 gene atf1, recombinant coexpression of the enzyme with genes YjgB, KDC, ARO8, SerA, ARoG, and TrpABCD from Saccharomyces cerevisiae strain YPH499 in Escherichia coli strain MG1655 to establish the production of indole-3-ethanol and indole-3-ethanol acetate from glucose, method, overview. Subcloning in Escherichia coli strain DH1013 755994 2.3.1.84 generation of transgenic petunia plants expressing the rose gene under the control of a CaMV-35S promoter 676539 2.3.1.84 into the pBAD-TOPO vector, furthermore a pTRC construct is used, expression experiments are performed in Escherichia coli cells 702712 2.3.1.84 overexpressed in Saccharomyces cerevisiae: Gas chromatographic analysis of small-scale fermentations with the transformant strain shows that KlATF overexpression only causes a moderate increase in acetate esters compared to Saccharomyces cerevisiae ATF1 overexpression results in a substantial increase in acetate ester levels 684642 2.3.1.84 overexpression of ATF1p-GFP fusion protein in Sacharomyces cerevisiae 663448