2.4.1.210 cDNA clone encoding limonoid UDP-glucosyltransferase isolated, single copy gene in the citrus genome 2.4.1.210 Citrus unshiu is heterozygous posxadsessing both alleles, CitLGT1/CitLGT2, two alleles (CitLGT1/ CitLGT2) of LGT at a single locus 2.4.1.210 CrLGT cloning of full-length gene from a cDNA library, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, cloning in Escherichia coli strain DH5alpha, RT-PCR enzyme expression analyxadsis 2.4.1.210 expression in Escherichia coli for sequencing 2.4.1.210 gene SLLGT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli and in Pichia pastoris 2.4.1.210 gene SLLGT, single copy gene encoding LGT 2.4.1.210 recombinant expression of the MBP-tagged enzyme (MBP-cuGT) in Escherichia coli strain BLR(DE3). MBP-cuGT does not exhibit any detectable effect on limonin degradation when Navel orange juice is used as the substrate. MBP-cuGT is unable to biotransform either limonoate A-ring lactone (LARL) or limonoate as purified substrates. But MBP-cuGT displays a broad activity spectrum towards flavonoids, confirming that the enzyme produced is active under the conditions evaluated in vitro, expression method evaluation, overview 2.4.1.210 single copy gene encoding LGT 2.4.1.210 single copy gene encoding LGT, Citrus sinensis is homozygous for allele CitLGT1