4.2.1.33 - 4.2.1.33 expressed in Escherichia coli BL21(DE3) cells 4.2.1.33 expression in Escherichia coli BL21(DE3) Star cells 4.2.1.33 expression of FLAG-tagged LeuC and of the three LeuD isoforms in transgenic Arabidosis thaliana plants, interaction analysis, overview 4.2.1.33 expression of His6-tagged LeuD in Escherichia coli strain BL21(DE3) 4.2.1.33 full length reading frame of IPMI small subunit 2 fused in frame to the 5' end of the gene encoding the green fluorescent protein in the vector psmGFP4. Construct transiently expressed in tobacco protoplasts and stably transformed into Arabidopsis plants 4.2.1.33 recombinant expression of GFP-tagged large and small subunit 1 in Arabidopsis thaliana leaves 4.2.1.33 variants LeuD-1-156 and LeuD-1-168, ligated into the pETM-11 expression vector, expressed in Escherichia coli BL21(DE3) RP