1.3.1.119 all open reading frames from the tcbAB cluster are cloned in the expression vector pET8c under transcriptional control of the T7 promoter in Escherichia coli BL21(DE3) 748143 1.3.1.119 expression in Escherichia coli DH5a(pTCB149) 746811 1.3.1.119 expression in Escherichia coli DH5alpha 748077 1.3.1.119 the tecB gene is cloned together with the tecA gene into Escherichia coli DH5alpha 746812