1.1.99.31 0.03 - (R,S)-p-chloromandelate mutant enzyme H274G, in presence of 20 mM imidazole 667566 1.1.99.31 0.03 - 2-Hydroxyisovalerate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 0.03 - O2 pH 7.5, 20°C, mutant enzyme MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with 205 residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667577 1.1.99.31 0.04 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R165G/R277K 667572 1.1.99.31 0.05 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme G81V 667577 1.1.99.31 0.05 - O2 pH 7.5, 20°C, mutant enzyme G81D 667577 1.1.99.31 0.07 - O2 pH 7.5, 20°C, mutant enzyme G81V 667577 1.1.99.31 0.09 - (S)-Mandelate mutant enzyme H274G, in presence of 20 mM imidazole 667566 1.1.99.31 0.1 - 2-Hydroxybutyrate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 0.1 - 2-Hydroxybutyrate pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 0.11 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme G81D 667577 1.1.99.31 0.15 - 3-Phenyllactate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 0.19 - 2-hydroxyhexanoate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 0.22 - O2 pH 7.5, 20°C, mutant enzyme, G81A/MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach and a G81A mutation in MDH 667577 1.1.99.31 0.23 - (S)-Mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 678128 1.1.99.31 0.23 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277G 667572 1.1.99.31 0.26 - 2-Hydroxyoctanoate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 0.27 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277L 667567 1.1.99.31 0.27 - 2-hydroxyvalerate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 0.27 - 3-indolelactate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 0.3 - 2-Hydroxyoctanoate mutant G81A 695329 1.1.99.31 0.3 - 3-indolelactate mutant G81A 695329 1.1.99.31 0.3 - ethyl-(S)-mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 678128 1.1.99.31 0.34 - 2-hydroxyhexanoate pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 0.36 - 2-hydroxyvalerate pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 0.37 - 2-Hydroxybutyrate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 0.42 - 2-Hydroxyoctanoate mutant G81A of chimera MDH-GOX2 695329 1.1.99.31 0.48 - 2-hydroxyhexanoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 0.48 - 2-hydroxyisocaproate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 0.5 - 2-Hydroxy-3-butenoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 0.5 - 2-Hydroxyoctanoate pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 0.5 - 2-Hydroxyoctanoate wild-type enzyme 695329 1.1.99.31 0.5 - 2-hydroxyvalerate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 0.52 - 3-Phenyllactate pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 0.61 - O2 pH 7.5, 20°C, mutant enzyme G81S 667577 1.1.99.31 0.64 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277G 667567 1.1.99.31 0.8 - 2-hydroxyisocaproate pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 0.87 - 3-Phenyllactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 0.96 - 2-Hydroxyoctanoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 1 - 2-Hydroxyoctanoate chimera MDH-GOX2 695329 1.1.99.31 1 - 3-indolelactate pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 1 - 3-indolelactate wild-type enzyme 695329 1.1.99.31 1.1 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R165K/R277K 667572 1.1.99.31 1.2 - indoleglycolate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 1.2 - O2 pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 1.28 - 2-hydroxyisocaproate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 1.4 - 3-indolelactate mutant G81A of chimera MDH-GOX2 695329 1.1.99.31 1.52 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277H 667567 1.1.99.31 1.6 - (S)-Mandelate pH 7.5, chimeric mutant of (S)-mandelate dehydrogenase with membrane anchoring loop replaced by a portion of glycolate oxidase from spinach 667564 1.1.99.31 2.3 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme, G81A/MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach and a G81A mutation in MDH 667577 1.1.99.31 2.3 - Mandelate mutant G81A of chimera MDH-GOX2 695329 1.1.99.31 2.3 - methyl-(S)-mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 678128 1.1.99.31 2.5 - methyl-(S)-mandelate mutant enzyme R277G, at 20°C, in 0.1 M potassium phosphate, pH 7.5 678128 1.1.99.31 2.6 - ethyl-(S)-mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 678128 1.1.99.31 2.8 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme G81S 667577 1.1.99.31 3 6 (S)-Mandelate pH 7.5, 25°C, electron acceptor: 2,6-dichloroindophenol 667455 1.1.99.31 3.13 - 3-indolelactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 3.4 - O2 pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 3.85 - 3-indolelactate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 3.9 - 2-Hydroxy-3-butynoate pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 3.9 - 3-indolelactate chimera MDH-GOX2 695329 1.1.99.31 4.4 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R165E 667572 1.1.99.31 6.6 - 2-Hydroxy-3-butynoate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 9 - (S)-Mandelate pH 7.5, (S)-mandelate dehydrogenase with residues 2-4 deleted 667564 1.1.99.31 9.3 - 4-chloromandelate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 13.2 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R165G 667572 1.1.99.31 14.8 - 2-Hydroxy-3-butynoate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 15.2 - Mandelate mutant G81A 695329 1.1.99.31 15.2 - Mandelate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 18.4 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R165M 667572 1.1.99.31 19.2 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme G81A 667577 1.1.99.31 24 - (S)-Mandelate pH 7.5, (S)-mandelate dehydrogenase with 17 residues deleted at the carboxy terminus 667564 1.1.99.31 38.5 - indoleglycolate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 49 - (S)-Mandelate pH 7.5, 25°C, electron acceptor: cytochrome c 667455 1.1.99.31 66 - (S)-Mandelate mutant enzyme R277K, at 20°C, in 0.1 M potassium phosphate, pH 7.5 678128 1.1.99.31 66 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277K 667572 1.1.99.31 68 - 3-methoxy-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide 667457 1.1.99.31 68 - 4-chloro-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide 667457 1.1.99.31 73 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R277K 667567 1.1.99.31 80 - 4-methyl-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide 667457 1.1.99.31 88 - DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme 667458 1.1.99.31 92 - DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate cytochrome, LMDH holoenzyme 667458 1.1.99.31 93 - DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide 667457 1.1.99.31 94 - D,L-Mandelate pH 7.5, 25°C, cosubstrate: ferricyanid 667457 1.1.99.31 98 - 4-fluoro-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide 667457 1.1.99.31 99 - DL-[2-2H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain 667458 1.1.99.31 106 - 4-methoxy-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide 667457 1.1.99.31 108 - 3-hydroxy-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide 667457 1.1.99.31 108 - 4-bromo-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide 667457 1.1.99.31 109 - (S)-Mandelate pH 7.5, 25°C, electron acceptor: ferricyanide 667455 1.1.99.31 114 - L-Mandelate pH 7.5, 25°C, cosubstrate: ferricyanide 667457 1.1.99.31 116 - 4-chloro-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide 667457 1.1.99.31 120 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R165K 667572 1.1.99.31 122 - indoleglycolate pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 134 - (S)-Mandelate pH 7.5, 4°C 667575 1.1.99.31 146 - 4-hydroxy-DL-mandelate pH 7.5, 25°C, cosubstrate: ferricyanide 667457 1.1.99.31 151 - methyl-(S)-mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 678128 1.1.99.31 155 - DL-[2-1H]-mandelate pH 7.5, 25°C, cosubstrate cytochrome c, LMDH holoenzyme 667458 1.1.99.31 174 - (S)-Mandelate pH 7.5, wild-type enzyme 667564 1.1.99.31 195 - Mandelate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 201 - (S)-3-phenyllactate pH 7.5, 20°C, mutant enzyme R277G 667567 1.1.99.31 204 - ethyl-(S)-mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 678128 1.1.99.31 205 - (S)-Mandelate pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 205 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with 205 residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667577 1.1.99.31 205 - Mandelate chimera MDH-GOX2 695329 1.1.99.31 225 - cytochrome c pH 7.5, 25°C 667458 1.1.99.31 270 - (S)-3-phenyllactate pH 7.5, 20°C, wild-type enzyme 667567 1.1.99.31 270 - (S)-Mandelate pH 7.5, 20°C, wild-type enzyme 667567 1.1.99.31 290 - (S)-Mandelate pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, wild-type enzyme 667565 1.1.99.31 300 - DL-[2-1H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, LMDH holoenzyme 667458 1.1.99.31 313 - 2-oxo-2-phenylacetate - 669259 1.1.99.31 316 - DL-[2-1H]-mandelate pH 7.5, 25°C, cosubstrate ferricyanide, L-MDH flavin domain 667458 1.1.99.31 334 - 4-chloromandelate pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 350 - Mandelate pH 7.5, 20°C, wild-type enzyme 667577 1.1.99.31 350 - Mandelate wild-type enzyme 695329 1.1.99.31 360 - (S)-Mandelate pH 7.5, 20°C 667575 1.1.99.31 360 - (S)-Mandelate pH 7.5, 20°C, wild-type enzyme 667572, 667577 1.1.99.31 360 - (S)-Mandelate wild type enzyme, at 20°C, in 0.1 M potassium phosphate, pH 7.5 678128 1.1.99.31 402 - (S)-Mandelate - 669259 1.1.99.31 550 - ferricyanide pH 7.5, 25°C 667458