1.5.3.5 0.1 - (S)-nicotine wild-type, pH 7.4, 22°C 764029 1.5.3.5 0.26 - (S)-6-hydroxynicotine pH 7.0, 25°C, mutant enzyme K287M 741943 1.5.3.5 1.14 - (S)-nicotine mutant R274A/Y311W/C417W, pH 7.4, 37°C 764029 1.5.3.5 1.44 - (S)-nicotine mutant Y311W, pH 7.4, 22°C 764029 1.5.3.5 2.03 - (S)-nicotine pH 7.0, 40°C 741686 1.5.3.5 2.5 - (S)-6-hydroxynicotine mutant R274A/Y311W/C417W, pH 7.4, 22°C 764029 1.5.3.5 2.5 - (S)-6-hydroxynornicotine pH 7.0, 25°C, mutant enzyme N166A 741943 1.5.3.5 2.65 - (S)-nicotine mutant R274A/Y311W/C417W, pH 7.4, 22°C 764029 1.5.3.5 4.8 - (S)-6-hydroxynicotine pH 7.0, 25°C, mutant enzyme N166A 741943 1.5.3.5 7.3 - (S)-6-hydroxynicotine pH 7.0, 40°C 741686 1.5.3.5 9 - (S)-6-hydroxynicotine mutant Y311W, pH 7.4, 22°C 764029 1.5.3.5 9.1 - (S)-6-hydroxynornicotine pH 7.0, 25°C, mutant enzyme Y311F 741943 1.5.3.5 16 - (S)-6-hydroxynornicotine pH 7.0, 25°C, wild-type enzyme 741943 1.5.3.5 20.9 - (S)-6-hydroxynicotine pH 7.0, 25°C, mutant enzyme Y311F 741943 1.5.3.5 25.8 - (S)-6-hydroxynicotine pH 8.0, 25°C 741691 1.5.3.5 29.3 - 6-hydroxy-L-nicotine - 392436, 392438 1.5.3.5 56 - (S)-6-hydroxynicotine wild-type, pH 7.4, 22°C 764029 1.5.3.5 78 - (S)-6-hydroxynicotine pH 7.0, 25°C, wild-type enzyme 741943