1.1.99.31 0.03 - (R,S)-p-chloromandelate mutant enzyme H274G, in presence of 20 mM imidazole 667566 1.1.99.31 0.03 - 2-Hydroxyisovalerate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 0.03 - O2 pH 7.5, 20°C, mutant enzyme MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with 205 residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667577 1.1.99.31 0.04 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme R165G/R277K 667572 1.1.99.31 0.05 - (S)-Mandelate pH 7.5, 20°C, mutant enzyme G81V 667577 1.1.99.31 0.05 - O2 pH 7.5, 20°C, mutant enzyme G81D 667577 1.1.99.31 0.07 - O2 pH 7.5, 20°C, mutant enzyme G81V 667577 1.1.99.31 0.09 - (S)-Mandelate mutant enzyme H274G, in presence of 20 mM imidazole 667566 1.1.99.31 0.1 - 2-Hydroxybutyrate pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach 667565 1.1.99.31 0.1 - 2-Hydroxybutyrate pH 7.5, 20°C, wild-type enzyme 667577