1.1.1.248 4.5 7.5 the enzyme shows a steep decrease by 90% of activity within 1.5 pH units on both sides of the optimum at pH 6.0 for the reduction reaction 1.1.1.248 4.7 7.5 sharp increase in activity at pH 4.7, half-maximal activity at pH 7.5, inactive at pH 9.0, recombinant enzyme, reduction reaction 1.1.1.248 5.8 7.8 50% of maximal activity at pH 5.8 and at pH 7.8, formation of (7S)-salutaridinol 1.1.1.248 8.5 10 50% of maximal activity at pH 5.8 and at pH 7.8, formationof salutaridine 1.1.1.248 9 10 maximal activity at pH 9.5, sharp decline in activity at pH 10.0, oxidation reaction, recombinant enzyme