3.1.1.1	carboxylesterase	-	
3.1.1.10	tropinesterase	-	
3.1.1.100	chlorophyllide a hydrolase	-	
3.1.1.101	poly(ethylene terephthalate) hydrolase	-	
3.1.1.102	mono(ethylene terephthalate) hydrolase	-	
3.1.1.103	teichoic acid D-alanine hydrolase	-	
3.1.1.104	5-phospho-D-xylono-1,4-lactonase	-	
3.1.1.105	3-O-acetylpapaveroxine carboxylesterase	-	
3.1.1.106	O-acetyl-ADP-ribose deacetylase	-	
3.1.1.107	apo-salmochelin esterase	-	
3.1.1.108	iron(III)-enterobactin esterase	-	
3.1.1.109	iron(III)-salmochelin esterase	-	
3.1.1.11	pectinesterase	-	
3.1.1.110	xylono-1,5-lactonase	-	
3.1.1.111	phosphatidylserine sn-1 acylhydrolase	-	
3.1.1.112	isoamyl acetate esteras	-	
3.1.1.113	ethyl acetate hydrolase	-	
3.1.1.114	methyl acetate hydrolase	-	
3.1.1.115	D-apionolactonase	-	
3.1.1.116	sn-1-specific diacylglycerol lipase	-	
3.1.1.117	(4-O-methyl)-D-glucuronate---lignin esterase	-	
3.1.1.118	phospholipid sn-1 acylhydrolase	-	
3.1.1.12	vitamin A esterase	deleted	
3.1.1.13	sterol esterase	-	
3.1.1.14	chlorophyllase	-	
3.1.1.15	L-arabinonolactonase	-	
3.1.1.16	4-carboxymethyl-4-hydroxyisocrotonolactonase	deleted, reaction is that of EC 5.3.3.4 and EC 3.1.1.24	
3.1.1.17	gluconolactonase	-	
3.1.1.18	aldonolactonase	deleted, included in EC 3.1.1.17	
3.1.1.19	uronolactonase	-	
3.1.1.2	arylesterase	-	
3.1.1.20	tannase	-	
3.1.1.21	retinyl-palmitate esterase	deleted, now covered by EC 3.1.1.1, carboxylesterase and EC 3.1.1.3, triacylglycerol lipase	
3.1.1.22	hydroxybutyrate-dimer hydrolase	-	
3.1.1.23	acylglycerol lipase	-	
3.1.1.24	3-oxoadipate enol-lactonase	-	
3.1.1.25	1,4-lactonase	-	
3.1.1.26	galactolipase	-	
3.1.1.27	4-pyridoxolactonase	-	
3.1.1.28	acylcarnitine hydrolase	-	
3.1.1.29	aminoacyl-tRNA hydrolase	-	
3.1.1.3	triacylglycerol lipase	-	
3.1.1.30	D-arabinonolactonase	-	
3.1.1.31	6-phosphogluconolactonase	-	
3.1.1.32	phospholipase A1	-	
3.1.1.33	6-acetylglucose deacetylase	-	
3.1.1.34	lipoprotein lipase	-	
3.1.1.35	dihydrocoumarin hydrolase	-	
3.1.1.36	limonin-D-ring-lactonase	-	
3.1.1.37	steroid-lactonase	-	
3.1.1.38	triacetate-lactonase	-	
3.1.1.39	actinomycin lactonase	-	
3.1.1.4	phospholipase A2	-	
3.1.1.40	orsellinate-depside hydrolase	-	
3.1.1.41	cephalosporin-C deacetylase	-	
3.1.1.42	chlorogenate hydrolase	-	
3.1.1.43	alpha-amino-acid esterase	-	
3.1.1.44	4-methyloxaloacetate esterase	-	
3.1.1.45	carboxymethylenebutenolidase	-	
3.1.1.46	Deoxylimonate A-ring-lactonase	-	
3.1.1.47	1-alkyl-2-acetylglycerophosphocholine esterase	-	
3.1.1.48	fusarinine-C ornithinesterase	-	
3.1.1.49	sinapine esterase	-	
3.1.1.5	lysophospholipase	-	
3.1.1.50	wax-ester hydrolase	-	
3.1.1.51	phorbol-diester hydrolase	-	
3.1.1.52	phosphatidylinositol deacylase	-	
3.1.1.53	sialate O-acetylesterase	-	
3.1.1.54	acetoxybutynylbithiophene deacetylase	-	
3.1.1.55	acetylsalicylate deacetylase	-	
3.1.1.56	methylumbelliferyl-acetate deacetylase	-	
3.1.1.57	2-pyrone-4,6-dicarboxylate lactonase	-	
3.1.1.58	N-acetylgalactosaminoglycan deacetylase	-	
3.1.1.59	juvenile-hormone esterase	-	
3.1.1.6	acetylesterase	-	
3.1.1.60	bis(2-ethylhexyl)phthalate esterase	-	
3.1.1.61	protein-glutamate methylesterase	-	
3.1.1.62	N-acetyldiaminopimelate deacylase	transferred to EC 3.5.1.47	
3.1.1.63	11-cis-retinyl-palmitate hydrolase	-	
3.1.1.64	retinoid isomerohydrolase	-	
3.1.1.65	L-rhamnono-1,4-lactonase	-	
3.1.1.66	5-(3,4-diacetoxybut-1-ynyl)-2,2'-bithiophene deacetylase	-	
3.1.1.67	fatty-acyl-ethyl-ester synthase	-	
3.1.1.68	xylono-1,4-lactonase	-	
3.1.1.69	N-acetylglucosaminylphosphatidylinositol deacetylase	transferred to EC 3.5.1.89	
3.1.1.7	acetylcholinesterase	-	
3.1.1.70	cetraxate benzylesterase	-	
3.1.1.71	acetylalkylglycerol acetylhydrolase	-	
3.1.1.72	acetylxylan esterase	-	
3.1.1.73	feruloyl esterase	-	
3.1.1.74	cutinase	-	
3.1.1.75	poly(3-hydroxybutyrate) depolymerase	-	
3.1.1.76	poly(3-hydroxyoctanoate) depolymerase	-	
3.1.1.77	acyloxyacyl hydrolase	-	
3.1.1.78	polyneuridine-aldehyde esterase	-	
3.1.1.79	hormone-sensitive lipase	-	
3.1.1.8	cholinesterase	-	
3.1.1.80	acetylajmaline esterase	-	
3.1.1.81	quorum-quenching N-acyl-homoserine lactonase	-	
3.1.1.82	pheophorbidase	-	
3.1.1.83	monoterpene epsilon-lactone hydrolase	-	
3.1.1.84	cocaine esterase	-	
3.1.1.85	pimelyl-[acyl-carrier protein] methyl ester esterase	-	
3.1.1.86	rhamnogalacturonan acetylesterase	-	
3.1.1.87	fumonisin B1 esterase	-	
3.1.1.88	pyrethroid hydrolase	-	
3.1.1.89	protein phosphatase methylesterase-1	-	
3.1.1.9	benzoylcholinesterase	deleted	
3.1.1.90	all-trans-retinyl ester 13-cis isomerohydrolase	-	
3.1.1.91	2-oxo-3-(5-oxofuran-2-ylidene)propanoate lactonase	-	
3.1.1.92	4-sulfomuconolactone hydrolase	-	
3.1.1.93	mycophenolic acid acyl-glucuronide esterase	-	
3.1.1.94	versiconal hemiacetal acetate esterase	-	
3.1.1.95	aclacinomycin methylesterase	-	
3.1.1.96	D-aminoacyl-tRNA deacylase	-	
3.1.1.97	methylated diphthine methylhydrolase	-	
3.1.1.98	[Wnt protein] O-palmitoleoyl-L-serine hydrolase	-	
3.1.1.99	6-deoxy-6-sulfogluconolactonase	-	
3.1.1.B10	p-coumaroyl esterase	preliminary BRENDA-supplied EC number	
3.1.1.B11	glucuronoyl esterase	deleted, replaced by EC 3.1.1.117	
3.1.1.B12	zearalenone hydrolase	preliminary BRENDA-supplied EC number	
3.1.1.B13	ergosteryl-3beta-O-L-aspartate hydrolase	preliminary BRENDA-supplied EC number	
3.1.11.1	exodeoxyribonuclease I	-	
3.1.11.2	exodeoxyribonuclease III	-	
3.1.11.3	exodeoxyribonuclease (lambda-induced)	-	
3.1.11.4	exodeoxyribonuclease (phage SP3-induced)	-	
3.1.11.5	exodeoxyribonuclease V	-	
3.1.11.6	exodeoxyribonuclease VII	-	
3.1.11.7	adenosine-5'-diphospho-5'-[DNA] diphosphatase	transferred to EC 3.6.1.71	
3.1.11.8	guanosine-5'-diphospho-5'-[DNA] diphosphatase	transferred to EC 3.6.1.70	
3.1.12.1	5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming)	-	
3.1.12.2	DNA-3'-diphospho-5'-guanosine diphosphatase	transferred to EC 3.6.1.72	
3.1.13.1	exoribonuclease II	-	
3.1.13.2	exoribonuclease H	-	
3.1.13.3	oligonucleotidase	-	
3.1.13.4	poly(A)-specific ribonuclease	-	
3.1.13.5	ribonuclease D	-	
3.1.13.B1	5'-3' exoribonuclease	preliminary BRENDA-supplied EC number	
3.1.14.1	yeast ribonuclease	-	
3.1.15.1	venom exonuclease	-	
3.1.15.B1	NrnC nuclease	preliminary BRENDA-supplied EC number	
3.1.16.1	spleen exonuclease	-	
3.1.2.1	acetyl-CoA hydrolase	-	
3.1.2.10	formyl-CoA hydrolase	-	
3.1.2.11	acetoacetyl-CoA hydrolase	-	
3.1.2.12	S-formylglutathione hydrolase	-	
3.1.2.13	S-succinylglutathione hydrolase	-	
3.1.2.14	oleoyl-[acyl-carrier-protein] hydrolase	-	
3.1.2.15	ubiquitin thiolesterase	deleted, activity due to EC 3.4.19.12, ubiquitinyl hydrolase 1	
3.1.2.16	citrate-lyase deacetylase	-	
3.1.2.17	(S)-methylmalonyl-CoA hydrolase	-	
3.1.2.18	ADP-dependent short-chain-acyl-CoA hydrolase	-	
3.1.2.19	ADP-dependent medium-chain-acyl-CoA hydrolase	-	
3.1.2.2	palmitoyl-CoA hydrolase	-	
3.1.2.20	acyl-CoA hydrolase	-	
3.1.2.21	dodecanoyl-[acyl-carrier-protein] hydrolase	-	
3.1.2.22	palmitoyl[protein] hydrolase	-	
3.1.2.23	4-hydroxybenzoyl-CoA thioesterase	-	
3.1.2.24	2-(2-hydroxyphenyl)benzenesulfinate hydrolase	transferred to EC 3.13.1.3	
3.1.2.25	phenylacetyl-CoA hydrolase	-	
3.1.2.26	bile-acid-CoA hydrolase	transferred to EC 2.8.3.25	
3.1.2.27	choloyl-CoA hydrolase	-	
3.1.2.28	1,4-dihydroxy-2-naphthoyl-CoA hydrolase	-	
3.1.2.29	fluoroacetyl-CoA thioesterase	-	
3.1.2.3	succinyl-CoA hydrolase	-	
3.1.2.30	(3S)-malyl-CoA thioesterase	-	
3.1.2.31	dihydromonacolin L-[lovastatin nonaketide synthase] thioesterase	-	
3.1.2.32	2-aminobenzoylacetyl-CoA thioesterase	-	
3.1.2.4	3-hydroxyisobutyryl-CoA hydrolase	-	
3.1.2.5	hydroxymethylglutaryl-CoA hydrolase	-	
3.1.2.6	hydroxyacylglutathione hydrolase	-	
3.1.2.7	glutathione thiolesterase	-	
3.1.2.8	S-acetoacylglutathione hydrolase	deleted, included in EC 3.1.2.6	
3.1.2.9	S-acetoacetylhydrolipoate hydrolase	deleted	
3.1.2.B5	3,4-hydroxyphenylacetyl-CoA thioesterase	preliminary BRENDA-supplied EC number	
3.1.21.1	deoxyribonuclease I	-	
3.1.21.10	crossover junction endodeoxyribonuclease	-	
3.1.21.2	deoxyribonuclease IV	-	
3.1.21.3	type I site-specific deoxyribonuclease	-	
3.1.21.4	type II site-specific deoxyribonuclease	-	
3.1.21.5	type III site-specific deoxyribonuclease	-	
3.1.21.6	CC-preferring endodeoxyribonuclease	-	
3.1.21.7	deoxyribonuclease V	-	
3.1.21.8	T4 deoxyribonuclease II	-	
3.1.21.9	T4 deoxyribonuclease IV	-	
3.1.21.B1	endonuclease Q	preliminary BRENDA-supplied EC number	
3.1.21.B2	restriction endonuclease PabI	preliminary BRENDA-supplied EC number	
3.1.21.B3	mismatch-specific endonuclease	preliminary BRENDA-supplied EC number	
3.1.22.1	deoxyribonuclease II	-	
3.1.22.2	Aspergillus deoxyribonuclease K1	-	
3.1.22.3	deoxyribonuclease V	transferred to EC 3.1.21.7	
3.1.22.4	crossover junction endodeoxyribonuclease	transferred to EC 3.1.21.10	
3.1.22.5	deoxyribonuclease X	-	
3.1.25.1	deoxyribonuclease (pyrimidine dimer)	-	
3.1.25.2	endodeoxyribonuclease (apurinic or apyrimidinic)	transferred to EC 4.2.99.18	
3.1.26.1	Physarum polycephalum ribonuclease	-	
3.1.26.10	ribonuclease IX	-	
3.1.26.11	tRNase Z	-	
3.1.26.12	ribonuclease E	-	
3.1.26.13	retroviral ribonuclease H	-	
3.1.26.2	ribonuclease alpha	-	
3.1.26.3	ribonuclease III	-	
3.1.26.4	ribonuclease H	-	
3.1.26.5	ribonuclease P	-	
3.1.26.6	ribonuclease IV	-	
3.1.26.7	ribonuclease P4	-	
3.1.26.8	ribonuclease M5	-	
3.1.26.9	ribonuclease [poly-(U)-specific]	-	
3.1.27.1	ribonuclease T2	transferred to EC 4.6.1.19	
3.1.27.10	rRNA endonuclease	transferred to EC 4.6.1.23	
3.1.27.2	Bacillus subtilis ribonuclease	transferred to EC 4.6.1.22	
3.1.27.3	ribonuclease T1	transferred to EC 4.6.1.24	
3.1.27.4	ribonuclease U2	transferred to EC 4.6.1.20	
3.1.27.5	pancreatic ribonuclease	transferred to EC 4.6.1.18	
3.1.27.6	Enterobacter ribonuclease	transferred to EC 4.6.1.21	
3.1.27.7	ribonuclease F	-	
3.1.27.8	Ribonuclease V	-	
3.1.27.9	tRNA-intron endonuclease	transferred to EC 4.6.1.16	
3.1.27.B1	pre-mRNA splicing endonuclease EndA	preliminary BRENDA-supplied EC number	
3.1.3.1	alkaline phosphatase	-	
3.1.3.10	glucose-1-phosphatase	-	
3.1.3.100	thiamine phosphate phosphatase	-	
3.1.3.101	validoxylamine A 7'-phosphate phosphatase	-	
3.1.3.102	FMN hydrolase	-	
3.1.3.103	3-deoxy-D-glycero-D-galacto-nonulopyranosonate 9-phosphatase	-	
3.1.3.104	5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase	-	
3.1.3.105	N-acetyl-D-muramate 6-phosphate phosphatase	-	
3.1.3.106	2-lysophosphatidate phosphatase	-	
3.1.3.107	amicoumacin phosphatase	-	
3.1.3.108	nocturnin	-	
3.1.3.11	fructose-bisphosphatase	-	
3.1.3.12	trehalose-phosphatase	-	
3.1.3.13	bisphosphoglycerate phosphatase	deleted, activity due to EC 5.4.2.11, phosphoglycerate mutase (2,3-diphosphoglycerate-dependent)
	
3.1.3.14	methylphosphothioglycerate phosphatase	-	
3.1.3.15	histidinol-phosphatase	-	
3.1.3.16	protein-serine/threonine phosphatase	-	
3.1.3.17	[phosphorylase] phosphatase	-	
3.1.3.18	phosphoglycolate phosphatase	-	
3.1.3.19	glycerol-2-phosphatase	-	
3.1.3.2	acid phosphatase	-	
3.1.3.20	phosphoglycerate phosphatase	-	
3.1.3.21	glycerol-1-phosphatase	-	
3.1.3.22	mannitol-1-phosphatase	-	
3.1.3.23	sugar-phosphatase	-	
3.1.3.24	sucrose-phosphate phosphatase	-	
3.1.3.25	inositol-phosphate phosphatase	-	
3.1.3.26	4-phytase	-	
3.1.3.27	phosphatidylglycerophosphatase	-	
3.1.3.28	ADP-phosphoglycerate phosphatase	-	
3.1.3.29	N-acylneuraminate-9-phosphatase	-	
3.1.3.3	phosphoserine phosphatase	-	
3.1.3.30	3'-phosphoadenylylsulfate 3'-phosphatase	deleted, included in EC 3.1.3.31	
3.1.3.31	nucleotidase	deleted. The activity may be that of an acid phosphatase.	
3.1.3.32	polynucleotide 3'-phosphatase	-	
3.1.3.33	polynucleotide 5'-phosphatase	-	
3.1.3.34	deoxynucleotide 3'-phosphatase	-	
3.1.3.35	thymidylate 5'-phosphatase	-	
3.1.3.36	phosphoinositide 5-phosphatase	-	
3.1.3.37	sedoheptulose-bisphosphatase	-	
3.1.3.38	3-phosphoglycerate phosphatase	-	
3.1.3.39	streptomycin-6-phosphatase	-	
3.1.3.4	phosphatidate phosphatase	-	
3.1.3.40	guanidinodeoxy-scyllo-inositol-4-phosphatase	-	
3.1.3.41	4-nitrophenylphosphatase	-	
3.1.3.42	[glycogen-synthase-D] phosphatase	-	
3.1.3.43	[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase	-	
3.1.3.44	[acetyl-CoA carboxylase]-phosphatase	-	
3.1.3.45	3-deoxy-manno-octulosonate-8-phosphatase	-	
3.1.3.46	fructose-2,6-bisphosphate 2-phosphatase	-	
3.1.3.47	[hydroxymethylglutaryl-CoA reductase (NADPH)]-phosphatase	-	
3.1.3.48	protein-tyrosine-phosphatase	-	
3.1.3.49	[pyruvate kinase]-phosphatase	-	
3.1.3.5	5'-nucleotidase	-	
3.1.3.50	sorbitol-6-phosphatase	-	
3.1.3.51	dolichyl-phosphatase	-	
3.1.3.52	[3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring)]-phosphatase	-	
3.1.3.53	[myosin-light-chain] phosphatase	-	
3.1.3.54	fructose-2,6-bisphosphate 6-phosphatase	-	
3.1.3.55	caldesmon-phosphatase	-	
3.1.3.56	inositol-polyphosphate 5-phosphatase	-	
3.1.3.57	inositol-1,4-bisphosphate 1-phosphatase	-	
3.1.3.58	sugar-terminal-phosphatase	-	
3.1.3.59	alkylacetylglycerophosphatase	-	
3.1.3.6	3'-nucleotidase	-	
3.1.3.60	phosphoenolpyruvate phosphatase	-	
3.1.3.61	inositol-1,4,5-trisphosphate 1-phosphatase	deleted	
3.1.3.62	multiple inositol-polyphosphate phosphatase	-	
3.1.3.63	2-carboxy-D-arabinitol-1-phosphatase	-	
3.1.3.64	phosphatidylinositol-3-phosphatase	-	
3.1.3.65	inositol-1,3-bisphosphate 3-phosphatase	transferred to EC 3.1.3.64	
3.1.3.66	phosphatidylinositol-3,4-bisphosphate 4-phosphatase	-	
3.1.3.67	phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase	-	
3.1.3.68	2-deoxyglucose-6-phosphatase	-	
3.1.3.69	glucosylglycerol 3-phosphatase	-	
3.1.3.7	3'(2'),5'-bisphosphate nucleotidase	-	
3.1.3.70	mannosyl-3-phosphoglycerate phosphatase	-	
3.1.3.71	2-phosphosulfolactate phosphatase	-	
3.1.3.72	5-phytase	-	
3.1.3.73	adenosylcobalamin/alpha-ribazole phosphatase	-	
3.1.3.74	pyridoxal phosphatase	-	
3.1.3.75	phosphoethanolamine/phosphocholine phosphatase	-	
3.1.3.76	lipid-phosphate phosphatase	-	
3.1.3.77	acireductone synthase	-	
3.1.3.78	phosphatidylinositol-4,5-bisphosphate 4-phosphatase	-	
3.1.3.79	mannosylfructose-phosphate phosphatase	-	
3.1.3.8	3-phytase	-	
3.1.3.80	2,3-bisphosphoglycerate 3-phosphatase	-	
3.1.3.81	diacylglycerol diphosphate phosphatase	transferred. Now EC 3.6.1.75	
3.1.3.82	D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-	
3.1.3.83	D-glycero-alpha-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-	
3.1.3.84	ADP-ribose 1''-phosphate phosphatase	-	
3.1.3.85	glucosyl-3-phosphoglycerate phosphatase	-	
3.1.3.86	phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase	-	
3.1.3.87	2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase	-	
3.1.3.88	5''-phosphoribostamycin phosphatase	-	
3.1.3.89	5'-deoxynucleotidase	-	
3.1.3.9	glucose-6-phosphatase	-	
3.1.3.90	maltose 6'-phosphate phosphatase	-	
3.1.3.91	7-methylguanosine nucleotidase	-	
3.1.3.92	kanosamine-6-phosphate phosphatase	-	
3.1.3.93	L-galactose 1-phosphate phosphatase	-	
3.1.3.94	D-galactose 1-phosphate phosphatase	-	
3.1.3.95	phosphatidylinositol-3,5-bisphosphate 3-phosphatase	-	
3.1.3.96	pseudouridine 5'-phosphatase	-	
3.1.3.97	3',5'-nucleoside bisphosphate phosphatase	-	
3.1.3.98	CDP-glycerol:N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol glycerophosphotransferase	transferred to EC 3.6.1.68	
3.1.3.99	IMP-specific 5'-nucleotidase	-	
3.1.3.B13	sn-2-glycerol-3-phosphate phosphatase	preliminary BRENDA-supplied EC number	
3.1.3.B14	archaetidylinositol phosphate phosphatase	preliminary BRENDA-supplied EC number	
3.1.3.B4	phosphatidylinositol-4-phosphate phosphatase	preliminary BRENDA-supplied EC number	
3.1.3.B8	mannosylglucosyl-3-phosphoglycerate phosphatase	preliminary BRENDA-supplied EC number	
3.1.3.B9	L-galactose 1-phosphate phosphatase	preliminary BRENDA-supplied EC number	
3.1.30.1	Aspergillus nuclease S1	-	
3.1.30.2	Serratia marcescens nuclease	-	
3.1.31.1	micrococcal nuclease	-	
3.1.4.1	phosphodiesterase I	-	
3.1.4.10	1-phosphatidylinositol phosphodiesterase	transferred to EC 4.6.1.13	
3.1.4.11	phosphoinositide phospholipase C	-	
3.1.4.12	sphingomyelin phosphodiesterase	-	
3.1.4.13	serine-ethanolaminephosphate phosphodiesterase	-	
3.1.4.14	[acyl-carrier-protein] phosphodiesterase	-	
3.1.4.15	adenylyl-[glutamate-ammonia ligase] hydrolase	transferred to EC 2.7.7.89	
3.1.4.16	2',3'-cyclic-nucleotide 2'-phosphodiesterase	-	
3.1.4.17	3',5'-cyclic-nucleotide phosphodiesterase	-	
3.1.4.18	phosphodiesterase II	transferred to EC 3.1.16.1	
3.1.4.19	oligonucleotidase	transferred to EC 3.1.13.3	
3.1.4.2	glycerophosphocholine phosphodiesterase	-	
3.1.4.20	exoribonuclease	transferred to EC 3.1.13.1	
3.1.4.21	single-stranded-nucleate endonuclease	transferred to EC 3.1.30.1	
3.1.4.22	ribonuclease I	transferred to EC 3.1.27.5	
3.1.4.23	ribonuclease II	transferred to EC 3.1.27.1	
3.1.4.24	endoribonuclease III	deleted	
3.1.4.25	exodeoxyribonuclease I	transferred to EC 3.1.11.1	
3.1.4.26	exodeoxyribonuclease II	deleted	
3.1.4.27	exodeoxyribonuclease II	transferred to EC 3.1.11.2	
3.1.4.28	exodeoxyribonuclease IV	transferred to EC 3.1.11.3	
3.1.4.29	oligodeoxyribonucleate exonuclease	deleted	
3.1.4.3	phospholipase C	-	
3.1.4.30	endodeoxyribonuclease	transferred to EC 3.1.21.2	
3.1.4.31	DNA 5'-dinucleotidohydrolase	transferred to EC 3.1.11.4	
3.1.4.32	endodeoxyribonuclease (ATP- and S-adenosylmethionine-dependent)	deleted	
3.1.4.33	endodeoxyribonuclease (ATP-hydrolysing)	deleted	
3.1.4.34	hybrid nuclease	deleted	
3.1.4.35	3',5'-cyclic-GMP phosphodiesterase	-	
3.1.4.36	1,2-cyclic-inositol-phosphate phosphodiesterase	transferred to EC 3.1.4.43	
3.1.4.37	2',3'-cyclic-nucleotide 3'-phosphodiesterase	-	
3.1.4.38	glycerophosphocholine cholinephosphodiesterase	-	
3.1.4.39	alkylglycerophosphoethanolamine phosphodiesterase	-	
3.1.4.4	phospholipase D	-	
3.1.4.40	CMP-N-acylneuraminate phosphodiesterase	-	
3.1.4.41	sphingomyelin phosphodiesterase D	-	
3.1.4.42	glycerol-1,2-cyclic-phosphate 2-phosphodiesterase	-	
3.1.4.43	glycerophosphoinositol inositolphosphodiesterase	-	
3.1.4.44	glycerophosphoinositol glycerophosphodiesterase	-	
3.1.4.45	N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase	-	
3.1.4.46	glycerophosphodiester phosphodiesterase	-	
3.1.4.47	variant-surface-glycoprotein phospholipase C	transferred to EC 4.6.1.14	
3.1.4.48	dolichylphosphate-glucose phosphodiesterase	-	
3.1.4.49	dolichylphosphate-mannose phosphodiesterase	-	
3.1.4.5	deoxyribonuclease	transferred to EC 3.1.21.1	
3.1.4.50	glycosylphosphatidylinositol phospholipase D	-	
3.1.4.51	glucose-1-phospho-D-mannosylglycoprotein phosphodiesterase	-	
3.1.4.52	cyclic-guanylate-specific phosphodiesterase	-	
3.1.4.53	3',5'-cyclic-AMP phosphodiesterase	-	
3.1.4.54	N-acetylphosphatidylethanolamine-hydrolysing phospholipase D	-	
3.1.4.55	phosphoribosyl 1,2-cyclic phosphate phosphodiesterase	-	
3.1.4.56	7,8-dihydroneopterin 2',3'-cyclic phosphate phosphodiesterase	-	
3.1.4.57	phosphoribosyl 1,2-cyclic phosphate 1,2-diphosphodiesterase	-	
3.1.4.58	RNA 2',3'-cyclic 3'-phosphodiesterase	-	
3.1.4.59	cyclic-di-AMP phosphodiesterase	-	
3.1.4.6	deoxyribonuclease II	transferred to EC 3.1.22.1	
3.1.4.60	pApA phosphodiesterase	-	
3.1.4.61	cyclic 2,3-diphosphoglycerate hydrolase	-	
3.1.4.7	micrococcal nuclease	transferred to EC 3.1.31.1	
3.1.4.8	Aspergillus oryzae ribonuclease	transferred to EC 3.1.27.3	
3.1.4.9	nucleate endonuclease	transferred to EC 3.1.30.2	
3.1.4.B4	UDP-alpha-D-glucose glucohydrolase	preliminary BRENDA-supplied EC number	
3.1.5.1	dGTPase	-	
3.1.5.B1	dNTPase	preliminary BRENDA-supplied EC number	
3.1.6.1	arylsulfatase (type I)	-	
3.1.6.10	chondro-6-sulfatase	-	
3.1.6.11	disulfoglucosamine-6-sulfatase	-	
3.1.6.12	N-acetylgalactosamine-4-sulfatase	-	
3.1.6.13	iduronate-2-sulfatase	-	
3.1.6.14	N-acetylglucosamine-6-sulfatase	-	
3.1.6.15	N-sulfoglucosamine-3-sulfatase	-	
3.1.6.16	monomethyl-sulfatase	-	
3.1.6.17	D-lactate-2-sulfatase	-	
3.1.6.18	glucuronate-2-sulfatase	-	
3.1.6.19	(R)-specific secondary-alkylsulfatase (type III)	-	
3.1.6.2	steryl-sulfatase	-	
3.1.6.20	S-sulfosulfanyl-L-cysteine sulfohydrolase	-	
3.1.6.21	linear primary-alkylsulfatase	-	
3.1.6.22	branched primary-alkylsulfatase	-	
3.1.6.3	glycosulfatase	-	
3.1.6.4	N-acetylgalactosamine-6-sulfatase	-	
3.1.6.5	sinigrin sulfohydrolase; myrosulfatase	deleted	
3.1.6.6	choline-sulfatase	-	
3.1.6.7	cellulose-polysulfatase	-	
3.1.6.8	cerebroside-sulfatase	-	
3.1.6.9	chondro-4-sulfatase	-	
3.1.6.B2	fucoidan-2-sulfatase	preliminary BRENDA-supplied EC number	
3.1.7.1	prenyl-diphosphatase	-	
3.1.7.10	(13E)-labda-7,13-dien-15-ol synthase	-	
3.1.7.11	geranyl diphosphate diphosphatase	-	
3.1.7.12	(+)-kolavelool synthase	-	
3.1.7.13	neryl diphosphate diphosphatase	-	
3.1.7.2	guanosine-3',5'-bis(diphosphate) 3'-diphosphatase	-	
3.1.7.3	monoterpenyl-diphosphatase	-	
3.1.7.4	sclareol cyclase	deleted, now covered by EC 4.2.1.133 and EC 4.2.3.141	
3.1.7.5	geranylgeranyl diphosphate diphosphatase	-	
3.1.7.6	farnesyl diphosphatase	-	
3.1.7.7	drimenol synthase	transferred to EC 4.2.3.194	
3.1.7.8	tuberculosinol synthase	transferred to EC 2.5.1.153	
3.1.7.9	isotuberculosinol synthase	transferred to EC 2.5.1.153	
3.1.8.1	aryldialkylphosphatase	-	
3.1.8.2	diisopropyl-fluorophosphatase	-	
3.1.99.B1	flap endonuclease-1	preliminary BRENDA-supplied EC number	
3.1.99.B2	XPF endonuclease	preliminary BRENDA-supplied EC number	
3.1.99.B3	Hef endonuclease	preliminary BRENDA-supplied EC number	
3.1.99.B4	DNase xthA	preliminary BRENDA-supplied EC number	
3.1.99.B5	endonuclease SSO2001	preliminary BRENDA-supplied EC number	
3.1.99.B6	endonuclease Bax1	preliminary BRENDA-supplied EC number