1.14.11.29	D137A	mutation eliminates prolyl hydroxylase activity of HPH-1	701219	
1.14.11.29	D315E	substitution of the Fe(II)-binding aspartate for a glutamate residue manifests significantly reduced Fe(II) binding, yet maintains catalytic activity with a 5fold faster reaction with O2	741852	
1.14.11.29	H135A	mutation eliminates prolyl hydroxylase activity of HPH-1	701219	
1.14.11.29	H196A	mutation eliminates prolyl hydroxylase activity of HPH-1	701219	
1.14.11.29	additional information	PDH2 knockout in NP cells lentiviral expression of shPHD2, phenotype, overview. TNF-alpha-dependent induction in expression of PHD3, SDC4, MMP13, and ADAMTS5, catabolic marker genes that are hallmark of disc degeneration, is significantly reduced in PHD2 silenced cells. Silencing of PHD2 significantly restores the TNF-alpha-mediated reduction in aggrecan sand collagen II	745336	
1.14.11.29	additional information	siRNA against PHD2 causes sufficient PHD2 knockdown in glioblastoma cell lines U-87MG, U-138MG, and U-343MG	744649	
1.14.11.29	additional information	the expression of PHD2 gene is silenced via specific siRNA (siPHD2) in the erythropoietin (EPO)-producing human HCC cell line HepG2. PHD2 knockdown causes a marked reduction of erythropoietin (EPO) production. HIF seems not to be involved in this process	744664	
1.14.11.29	R367K	inactive mutant of HIF-P4H-1	659235	
1.14.11.29	R383K	disruption of 2OG binding in this variant does not accelerate O2 activation	741852	
1.14.11.29	T387A	the mutant shows 15fold increased turnover at limiting O2 concentrations compared to the wild type enzyme	741910	
1.14.11.29	T387N	the mutant shows about 2fold reduced turnover at limiting O2 concentrations compared to the wild type enzyme	741910