1.14.11.51	C118A	m6A demethylase activity similar to wild-type, about 25% decrease in apurinic/apyrimidinic lyase activity	764135	
1.14.11.51	C118A/C129A	about 50% decrease in m6A demethylase activity, about 20% decrease in apurinic/apyrimidinic lyase activity	764135	
1.14.11.51	C129A	about 90% decrease in m6A demethylase activity, about 30% decrease in apurinic/apyrimidinic lyase activity	764135	
1.14.11.51	D186A	inactive	742235	
1.14.11.51	D233A	no residual m6A demethylase activity, about 30% decrease in apurinic/apyrimidinic lyase activity	764135	
1.14.11.51	D233A	reduced activity of the recombinant mutant protein	736883	
1.14.11.51	D233A	residue plays an important role in substrate recognition and catalysis	764114	
1.14.11.51	H113A/C118A/C129A/H134A	no residual m6A demethylase activity, about 55% decrease in apurinic/apyrimidinic lyase activity	764135	
1.14.11.51	H231A	no residual m6A demethylase activity, about 20% decrease in apurinic/apyrimidinic lyase activity	764135	
1.14.11.51	H231A	residue plays an important role in substrate recognition and catalysis	764114	
1.14.11.51	H287A	no residual m6A demethylase activity, about 25% decrease in apurinic/apyrimidinic lyase activity	764135	
1.14.11.51	H287A	residue plays an important role in substrate recognition and catalysis	764114	
1.14.11.51	K133A	about 50% decrease in m6A demethylase activity, about 45% decrease in apurinic/apyrimidinic lyase activity	764135	
1.14.11.51	K158A	mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1	764442	
1.14.11.51	R159A	mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1	764442	
1.14.11.51	R160A	mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1	764442	
1.14.11.51	R167A	mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1	764442	
1.14.11.51	R169A	mutation of basic residue of Flip1 region, abolishes N6-mA demethylation activity of ALKBH1	764442	
1.14.11.51	R338A	residue plays an important role in substrate recognition and catalysis	764114	
1.14.11.51	R344A	mutation in highly conserved residue, strong decrease in demethylation ability	764114	
1.14.11.51	S235D	mutation disrupts N6-mA demethylation activity	764442	
1.14.11.51	S235E	mutation disrupts N6-mA demethylation activity	764442	
1.14.11.51	Y184A	mutation in highly conserved residue, strong decrease in demethylation ability	764114