1.4.1.13 C47A beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme 1.4.1.13 C50A beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme 1.4.1.13 C55A beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme 1.4.1.13 C59A beta subunit, no significant effect on NADPH:iodonitrotetrazolium oxidoreductase activity of subunit, complete loss of activity of enzyme 1.4.1.13 G298A mutant with an approximately 10fold decrease of the affinity of the enzyme for pyridine nucleotides with little or no effect on the rate of the enzyme reduction by NADPH, maintains the ability to bind NADPH and FAD, is catalytically active in the NADPH:iodonitrotetrazolium oxidoreductase reaction and has a monomeric state, mutation leads to production of insoluble protein under conditions that yield large amounts of soluble wild-type enzyme and to production of smaller amounts of enzyme