1.7.1.B2	E72Q	the mutation does not increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	E72Q/F108Y	the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	F108Y	the mutation substantially increases the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	F123A	site-directed mutagenesis, residue F123 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme	743709	
1.7.1.B2	F124A	site-directed mutagenesis, residue F124 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme	743709	
1.7.1.B2	F124N	site-directed mutagenesis, residue F124 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme	743709	
1.7.1.B2	F124W	site-directed mutagenesis, residue F124 is located in helix alpha6 constituting the substrate entry site, the mutant enzyme kinetics are altered compared to the wild-type enzyme	743709	
1.7.1.B2	F70A	the mutation substantially increases the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	F70A/E72Q	the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	F70A/E72Q/F108Y	the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	F70A/F108Y	the mutations confer high levels of 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide activity and also substantially improve activity with 5-(aziridin-1-yl)-2,4-dinitrobenzamide and metronidazole. The mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	F70A/F108Y	the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	N71S	site-directed mutagenesis, active site residue mutation, the mutant shows increased activity with prodrug 5-[aziridin-1-yl]-2,4-dinitrobenzamide (CB1954) and nitrofurazone compared to the wild-type enzyme	743709	
1.7.1.B2	T41L	site-directed mutagenesis, active site residue mutation, the mutant shows increased activity with prodrug 5-[aziridin-1-yl]-2,4-dinitrobenzamide (CB1954) and nitrofurazone compared to the wild-type enzyme	743709	
1.7.1.B2	T41L	the mutation does not increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	T41L/F70A	the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	T41L/F70A/F108Y	the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	T41L/F70A/N71S	the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	T41L/F70A/N71S/F108Y	the mutations substantially increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166	
1.7.1.B2	T41L/N71S	the mutations do not increase the sensitivity of the Escherichia coli host cell to 2-(2-methyl-5-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide	764166