2.7.8.11	C148W	polymorphism of unclear relevance, amplified from plant cDNA, mismatch with annotated genomic sequence	690824	
2.7.8.11	H114Q	oligonucleotide-mediated site-directed mutagenesis, substitution of amino acid at position 114 from His (CAC) + Gln (CAA) results in a 200fold increase in Km of the enzyme for myo-inositol, making cells auxotrophic for myo-inositol	645318	
2.7.8.11	Hy3	hybrid construct of 57 N-terminal amino acids from Saccharomyces cerevisiae PIS and 159 C-terminal amino acids from PfPIS, no in vitro activity, 60% complementation of in vivo activity in yeast lacking endogenous PIS gene compared to ScPIS complementation efficiency (100%)	677090	
2.7.8.11	Hy4	hybrid construct of 14 N-terminal amino acids from Saccharomyces cerevisiae PIS and 202 C-terminal amino acids from PfPIS, 37% complementation of in vivo activity in yeast lacking endogenous PIS gene compared to ScPIS complementation efficiency (100%)	677090	
2.7.8.11	K65C	site-directed mutagenesis, cysteine installation for substituted cysteine analysis method, SCAM	-, 737766	
2.7.8.11	additional information	an addition of a KXKXX motif reduces the activity of Pis1 by a factor of 2	-, 737766	
2.7.8.11	additional information	an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Homo sapiens cells. The recombinant cells do not show phosphatidylinositol phosphate synthase or archaetidylinositol phosphate synthase activities. Phosphatidylinositol synthase and archaetidylinositol synthase activities are determined, 7 pmol/h/mg and 2 pmol/h/mg, respectively	726887	
2.7.8.11	additional information	an artificial gene construct for a protein homologous to phosphatidylinositol phosphate synthase of Mycobacterium tuberculosis and archaetidylinositol phosphate synthase of Methanothermobacter thermautotrophicus is cloned in Escherichia coli and introduced into Saccharomyces cerevisiae cells. The recombinant cells do not show phosphatidylinositol phosphate synthase or archaetidylinositol phosphate synthase activities. Phosphatidylinositol synthase and archaetidylinositol synthase activities are determined, 107 pmol/h/mg and 7 pmol/h/mg, respectively	726887	
2.7.8.11	additional information	conditional double knockout mutant in the bloodstream form of Trypanosoma is not viable under nonpermissive conditions, which lead to a decrease in the amount of enzyme protein	671861	
2.7.8.11	additional information	gene successfully complements a Saccharomyces cerevisiae PIS1 deletion mutant	677090	
2.7.8.11	additional information	generation of transgenic maize constitutively overexpressing or underexpressing gene ZmPIS, encoding PIS from maize, under the control of a maize ubiquitin promoter. Lipid profiling analysis shows that, under drought stress conditions, the overexpression of ZmPIS in maize results in significantly elevated levels of most phospholipids and galactolipids in leaves compared with those in wild-type plants, analysis of expression pattern of genes involved in the phospholipid metabolism pathway and drought response, phenotype, overview. Sense transgenic plants are more tolerant to drought stress at the pre-flowering stage and retain more solutes and water under drought stress conditions	-, 739273	
2.7.8.11	N67C	site-directed mutagenesis, cysteine installation for substituted cysteine analysis method, SCAM	-, 737766	
2.7.8.11	S111C	missense mutation leads to lens opaque (lop) phenotype. Mutation is located in the catalytic domain of the 213 amino acid phosphatidylinositol (PI) synthase enzyme	724923