1.5.3.10	D552A	mutation leads to increased formaldehyde release	698911	
1.5.3.10	H225Q	modest changes in kinetic parameters, no stabilization of FADH2-iminium charge-transfer complex	672060	
1.5.3.10	H225Q	site-directed mutagenesis, reduced catalytic activity compared to the wild-type enzyme	655336	
1.5.3.10	additional information	complete removal of 5,10-CH2-THF synthase domain through expression of a truncated version generates a mutant enzyme unable to avoid hydrolysis of the imine species generated following amine oxidation	698911	
1.5.3.10	Y259F	able to oxidize substrate, steady-state turnover is attenuated 200fold, rate of FAD reduction is substantially impaired, no stabilization of FADH2-iminium charge-transfer complex	672060	
1.5.3.10	Y259F	site-directed mutagenesis, reduced catalytic activity compared to the wild-type enzyme	655336