Contents of a BRENDA enzyme entry.
This is part of the BRENDA json download file.
Unique identifier of the enzyme. For enzymes, the Enzyme Commission number (EC Number) given by the IUBMB (International Union of Biochemistry and Molecular Biology), classes of enzymes und subclasses based on the reaction they catalyze. For spontaneous, non-enzymatic reactions the id is spontaneous.
^(?:[1-7]\.[1-9][0-9]{0,2}\.[1-9][0-9]{0,2}\.B?[1-7][0-9]{0,2})|spontaneous$ The recommended or accepted name of an enzyme as given by the IUBMB.
The systematic name fields provides a chemically more precise name and is usaually based on the substrate or, in the case of a bimolecular reaction, of the two substrates separated by a colon, and the nature of the reaction.
Synonyms comprise names by which an enzyme is known. The names are found found in other databases or in the literature, abbreviations, names of commercially available products or gene names.
Actual or possible application in the fields of i.e. pharmacology, medicine, biofuel production, analysis, biotechnology etc. are described.
According to the type of reaction, the enzymes catalyze, enzymes are classified into 7 main classes, oxidoreductases, transferases, hydrolases, lyases, isomerases, ligases, and translocases with several subcategories, i.e. addition, elimination, carboxylation etc.
Information on the X-ray structure of an enzyme and the procedure and conditions of the crystallization are described.
Information on the renaturation, refolding and reactivation procedures of enzymes after denaturation processes.
General information on enzyme stability.
The influence of oxidating agents on enzyme stability.
The influence of storage duration and conditions such as temperature, pH, additives etc. on the enzyme stability and activity.
A generalized form of reactions catalyzed by the enzyme.
The reaction catalyzed by the enzyme under physiological conditions.
The reaction catalyzed by the enzyme.
The rate at which an enzyme converts its substrate in 1/s.
The Michaelis-Menten constant of the enzyme in mM.
The pH where the enzymes activity is at its maximum.
pH range in which the enzyme is active.
The pH stability of the enzyme.
The specific activity of the enzyme in µmol/min/mg.
The temperature where the enzymes activity is at its maximum in °C.
Temperature range in which the enzyme is active in °C.
The temperature stability of the enzyme in °C.
The molecular weight of the holoenzyme. For monomeric enzymes the MW is identical to the value given for subunits.
The pH value at which the protein carries no net electrical charge.
The inhibition constant in mM. Each value is connected to an inhibitor (value property).
The half maximal inhibitory concentration of a compund in mM. Each value is connected to an inhibitor (value property).
The catalytic efficiency of the enzyme in mM/s. Each value is connected to a substrate (value property).
The intracellular localization of the enzyme.
For multicellular organisms the tissues used for isolation of the enzyme or the tissue in which the enzyme is present. Cell-lines can also be a source of enzymes.
Compounds that have a positive effects on the activity of the enzyme, except metal ions and cofactors.
Compounds found to be inhibitory to the enzyme.
The ions or salts that have activating effects, or are closely bound to the enzyme.
This field describes covalent and general enzyme modifications following protein biosynthesis.
The tertiary structure of the active enzyme is described. It can be active as a monomer, dimer, trimer etc. The stoichiometry of the subunits is given.
All compounds which participate in the reaction, which are loosely-bound to the protein and are required for the enzyme activity, whereas prosthetic groups are tightly-bound to the enzyme.
Information on expresssion and cloning procedures and systems are given and in which organism or cell culture an enzyme is expressed in.
The enzyme stability in presence of organic solvents is described.
This information field describes the effect of compounds and/or conditions on the expression of enzymes leading to an up- or downregulation.
This field contains more general information on the role of an enzyme in the metabolism, the physiological function, a possible malfunction, or evolutionary aspects.
Protein/gene accessions associated with the data field.
All properties whose name matches the following regular expression must respect the following conditions
Property name regular expression:^[1-9][0-9]{0,}$ Must contain a minimum of 1 items
Must contain a minimum of 1 items
The references associated with the data field.
All properties whose name matches the following regular expression must respect the following conditions
Property name regular expression:^[1-9][0-9]{0,}$ [1-9][0-9]{0,} Value must be greater or equal to 1600
The organisms associated with the data field.
All properties whose name matches the following regular expression must respect the following conditions
Property name regular expression:^[1-9][0-9]{0,}$