EC Number   |
Activating Compound |
Reference |
|---|
    1.12.98.1 | ammonium sulfate |
improves reactivation of enzyme with FAD after FAD depletion |
395083 |
| 1.12.98.1 | FAD |
reactivation without added FAD restores only methyl viologen-dependent activity, no activity with F420 |
395093 |
| 1.12.98.1 | FAD |
restores activity after depletion of FAD in hydrophobic interaction chromatography |
395083, 395085 |
| 1.12.98.1 | KCl |
0.8 M activate F420-linked activity more than 10fold, slight inhibition of methyl viologen linked activity |
395093 |
| 1.12.98.1 | more |
2 times higher activity in biological buffers like Tris or EPPS compared with phosphate buffer |
395095 |
| 1.12.98.1 | more |
conditions for anaerobic reductive activation in the presence of hydrogen, 2-mercaptoethanol and KCl or methyl viologen yield maximal hydrogenase activity |
727399 |
| 1.12.98.1 | Procion Blue HB |
stimulates methyl viologen reduction |
395089 |
