EC Number   |
Activating Compound |
Reference |
|---|
   1.13.12.24 | 2-mercaptoethanol |
reuired. About 1% residual activity in absence of 2-mercaptoethanol |
746328 |
| 1.13.12.24 | dithiothreitol |
in the presence of DTT, continuous luminescence is observed over 1 h. In the absence of DTT, the luminescence activity slowly decreases with a half period of 8.3 min |
744889 |
| 1.13.12.24 | imidazole |
luminescence activity of BFP-aq 1s stimulated 1.5- to 2fold by imidazole at concentrations of 30-300 mM. Imidazole has no effect on the binding affinity of coelenterazine and may act as a catalytic base |
744216 |
| 1.13.12.24 | more |
1H-pyrazole, 2-methylimidazole, 4-methylimidazole, histamine, L-histidine, urocanic acid, and 5-amino-4-imidazole carboxamide do not act as stimulators at concentrations of 30 and 150 mM |
744216 |
