EC Number   |
Activating Compound |
Reference |
|---|
   7.2.2.8 | cardiolipin |
the hydrolytic activity of CopA is stimulated by phosphoholipid cardiolipin and to some extent by phosphatidylglycerol. The amphipathic platform helix MBC of CopA shows a significant preference for interaction with cardiolipin |
749929 |
| 7.2.2.8 | Cys |
millimolar concentration of Cys increase the activity about 800fold. Cys is a non-essential activator of the enzyme, interacting with the cytoplasmic side of the enzyme. K1/2 of 4 mM |
721700 |
| 7.2.2.8 | Insulin |
insulin reverses the effect of copper and stimulates retrograde trafficking of ATP7B from the canalicular membranes, consistent with the enhanced ability of ATP7B to sequester copper away from the cytosol. Physiological concentrations of insulin increase endogenous ATP7B activity in cultured hepatic cells and in tissues by 40%, whereas glucagon inhibits this activity by 70%. The opposite effects of the hormones on ATP7B activity involve receptor-mediated signaling pathways and membrane-bound kinases PKA and PKB/Akt |
749966 |
| 7.2.2.8 | L-cysteine |
isoform CtrA2 shows highest activity at 20 mM L-cysteine, isoform CtrA3 shows highest activity at 15 mM L-cysteine |
688411 |
| 7.2.2.8 | more |
dithiothreitol is necessary in the medium to maintain the metal in its Cu(I) redox state |
656109 |
| 7.2.2.8 | more |
isoform CtrA2 is activated without added lipid, whereas the activity of isoform CtrA3 depended on the addition of extraneous lipid. Escherichia coli polar lipids, soybean phosphatidyl choline, synthetic 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and a total extract of Aquifex aeolicus lipids all have roughly the same effect |
688411 |
| 7.2.2.8 | more |
metal-binding domain copper-loaded MBD1 serves CopA in a chaperone-like fashion, copper-loaded metal-binding domain MBD2 shows negligible stimulation |
734725 |
| 7.2.2.8 | NaCl |
highest activity at about 400 mM NaCl |
688411 |
| 7.2.2.8 | phosphatidylglycerol |
the hydrolytic activity of CopA is stimulated by phosphoholipid cardiolipin and to some extent by phosphatidylglycerol |
749929 |
| 7.2.2.8 | Phospholipid |
addition of phospholipids leads to a significant increase in CopA phosphomonoesterase turnover |
749955 |
