EC Number   |
General Stability |
Reference |
|---|
    1.6.1.2 | cations prevent from tryptic inactivation, 95% protection with Mn2+, 89% with Ca2+ and 79% with Mg2+ |
392634 |
| 1.6.1.2 | inactivation during prolonged column chromatography |
392637 |
| 1.6.1.2 | solubilized membrane component is unstable loosing all activity when stored overnight at 4°C or -70°C, stable at -70°C in the presence of 0.025 mM NADP+ for several months, inactivation by refreezing after thawing |
392641 |
| 1.6.1.2 | trypsinolysis is stimulated several fold by NADPH and NADP+, half-maximal stimulation with 0.001-0.002 mM NADPH and 0.002-0.003 mM NADP+, Mg2+ protects from NADP+ stimulated inactivation |
392641, 392647 |
| 1.6.1.2 | unstable, loses 30-50% activity within 48 h at 4°C, remainder of the enzyme becomes inactive after 2 weeks, complete inactivation after freezing at -20°C or -70°C overnight, enzyme is very susceptible to trypsinolysis, NADH protects from tryptic inactivation, NADPH promotes tryptic inactivation |
392640 |
| 1.6.1.2 | urea, 6 M, inactivation |
392631 |
