EC Number |
General Stability |
Reference |
---|
2.7.7.41 | -20°C, enzyme is very unstable when the disrupted lymphocyte homogenate or the isolated microsomes are stored, 75% loss of activity after 24 h. Stable for at least 1 month if stored in intact lymphocytes |
643311 |
2.7.7.41 | solubilized enzyme is stable to freezing and thawing |
643314 |
2.7.7.41 | stable to at least 2 cycles of freezing and thawing |
643301, 643303, 643309 |
2.7.7.41 | the pure enzyme can be dialyzed, but to maintain activity the dialyzing buffer must contain 1% n-octyl-beta-D-glucopyranoside, 0.25 mM dithiothreitol and at least 200 mM salt |
643300 |
2.7.7.41 | the solubilized protein is quite labile. Its stability is markedly enhanced by exchange dialysis against 10% glycerol, 20% propylene glycol, 25 mM cacodylate buffer, pH 6.5, 10 mM MgCl2 |
643310 |