EC Number |
Inhibitors |
Structure |
---|
7.1.1.1 | N,N'-dicyclohexylcarbodiimide |
modification of the enzyme with N,N'-dicyclohexylcarbodiimide leads to inhibition of the rate of release of NADP+ and NADPH from the enzyme, but has a much smaller effect on the binding and release of NAD+, NADH and their analogues and on the interconversion of the ternary complexes of the enzyme with its substrates |
|
7.1.1.1 | N,N'-Dicylclohexylcarbodiimide |
NADH protects from inhibition, NADP+ and to a lesser extent NADPH increase the rate of inhibition |
|
7.1.1.1 | N-cyclohexyl-N'-(4-dimethylaminonaphthyl)carbodiimide |
about 25% residual activity after 200 min at 0.5 mM |
|
7.1.1.1 | N-ethylmaleimide |
cysteine-free mutant enzyme is not inhibited |
|
7.1.1.1 | N-ethylmaleimide |
0.2 mM, 57% inhibition of reverse reaction in A348C mutant enzyme, 60% inhibition of K424C mutant enzyme, more than 95% inhibition of A390C mutant enzyme after 1h, R425C mutant enzyme is not inhibited |
|
7.1.1.1 | N-ethylmaleimide |
- |
|
7.1.1.1 | NAD(P)+ |
- |
|
7.1.1.1 | NADH |
high concentrations of NADH lead to substrate inhibition |
|
7.1.1.1 | NADP+ |
mixed product inhibition vs. acetylpyridine adenine dinucleotide, competitive vs. NADPH |
|
7.1.1.1 | NADPH |
- |
|