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EC Number
Inhibitors
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Structure
3.2.1.168
DMSO
enzyme hydrolytic activity is not modified by addition of 2% v/v DMSO and is significantly diminished at DMSO concentration of 10% v/v. After 18 h incubation the yield of product is nervertheless increased by 2fold; hydrolytic activity tested with 1.8 mM hesperidin as substrate. Activity is not modified by addition of 2% (v/v) DMSO, but is significantly diminished when the concentration is increased up to 10% (v/v). Hydrolysis yield is increased upon addition of DMSO, with hesperetin final concentrations in the range of 0.47-0.95 mM after 18 h reaction
3.2.1.168
DMSO
1 h incubation at 60°C, no loss of activity at 10% v/v DSMO, 40% loss at 20% v/v DSMO, and no activity at 50% v/v DSMO of the free enzyme. The immobilized enzyme retains 82% of the activity, independently of DSMO concentration after the exposure at high temperature
3.2.1.168
EDTA
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3.2.1.168
Mg2+
-
3.2.1.168
Zn2+
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