EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    4.2.99.20 | 0.0194 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
K212A mutant protein |
702326 |
| 4.2.99.20 | 0.0237 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
W147A mutant protein |
702326 |
| 4.2.99.20 | 0.028 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
S86A mutant protein |
702326 |
| 4.2.99.20 | 0.028 |
- |
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
mutant S86A at 25°C |
685225 |
| 4.2.99.20 | 0.028 |
- |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate |
25°C, mutant enzyme S86A |
685225 |
| 4.2.99.20 | 0.036 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
mutant Y148F, pH 7.0, temperature not specified in the publication |
730042 |
| 4.2.99.20 | 0.05 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
mutant Y148A, pH 7.0, temperature not specified in the publication |
730042 |
| 4.2.99.20 | 0.07 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
mutant F153A, pH 7.0, temperature not specified in the publication |
730042 |
| 4.2.99.20 | 0.074 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
W147F mutant protein |
702326 |
| 4.2.99.20 | 0.084 |
- |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate |
R124A mutant protein |
702326 |
