-999
-
NADH
Km-value for NADH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
0.015
-
NADH
mutant V48L/R49P/K52L/S53D/E59K/T182S/E320K, pH not specified in the publication, temperature not specified in the publication
0.024
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
0.026
-
NADH
mutant A71S/R76D/S78D/Q110V/D146G/G185R/K433E , pH not specified in the publication, temperature not specified in the publication
0.03
-
NADH
mutant A71S/R76D/S78D/Q110V, pH 7.0, temperature not specified in the publication
0.03
-
NADH
mutant A71S/R76D/S78D/Q110V, pH not specified in the publication, temperature not specified in the publication
0.039
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
0.04
-
NADH
mutant A71S/R76D/S78D/Q110A, pH 7.0, temperature not specified in the publication
0.045
-
NADH
wild-type, pH not specified in the publication, temperature not specified in the publication
0.047
-
NADH
mutant S61D/S63D/I95V, pH not specified in the publication, temperature not specified in the publication