-999
-
more
kinetic studies
-999
-
more
no activity with 5-40 mM sugars or hydroxyacids like 2-keto-L-guluonate, 3-deoxyglucosone, xylitol, L-threitol, ascorbic acid, malic acid, L-threonine, L-glycerate, L-tartarate and L-lactate, or 50 mM ethyl and methyl esters of 3-hydroxybutyrate
0.0005
-
NADH
at pH 8.5, rabbit liver GDH
0.0006
-
NADH
at pH 8.5, recombinant rabbit GDH
0.01
-
NAD+
at pH 8.5, recombinant rabbit GDH
0.01
-
NAD+
wild-type enzyme, pH 7.0, 25°C
0.011
-
NAD+
at pH 8.5, rabbit liver GDH
0.018
-
L-gulonate
with NAD+, wild-type enzyme, pH 7.0, 25°C
0.028
-
NAD+
mutant enzyme Q41N, pH 7.0, 25°C
0.04
-
NAD+
cosubstrate L-gulonate, pH 8.5
