EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.1.99.B3 | 0.3 |
- |
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose |
wild type enzyme, at pH 8.0 and 37°C |
740204 |
1.1.99.B3 | 52 |
- |
6-deoxy-D-glucose |
O2 used as electron acceptor |
685689 |
1.1.99.B3 | 75 |
- |
6-deoxy-D-glucose |
2,6-dichlorophenol indophenol used as electron acceptor |
685689 |
1.1.99.B3 | 0.078 |
- |
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose |
wild type enzyme, at pH 8.0 and 37°C |
740204 |
1.1.99.B3 | 0.09 |
- |
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose |
mutant enzyme A38V, at pH 8.0 and 37°C |
740204 |
1.1.99.B3 | 0.052 |
- |
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose |
mutant enzyme A38V, at pH 8.0 and 37°C |
740204 |
1.1.99.B3 | 0.059 |
- |
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose |
wild type enzyme, at pH 8.0 and 37°C |
740204 |
1.1.99.B3 | 0.039 |
- |
cellobiose |
mutant enzyme A38V, at pH 8.0 and 37°C |
740204 |
1.1.99.B3 | 0.04 |
- |
cellobiose |
wild type enzyme, at pH 8.0 and 37°C |
740204 |
1.1.99.B3 | 0.05 |
- |
cellobiose |
mutant enzyme A38V/S388N, at pH 8.0 and 37°C |
740204 |