EC Number   |
KM Value [mM]   |
KM Value Maximum [mM]   |
Substrate   |
Reference   |
---|
1.14.13.113 | 0.024 |
- |
NADH |
in 50 mM K2HPO4/NaH2PO4, pH 8.0, at 25°C |
727370 |
1.14.13.113 | 0.029 |
- |
Urate |
in 50 mM K2HPO4/NaH2PO4, pH 8.0, at 25°C |
727370 |
1.14.13.113 | 0.042 |
- |
Urate |
pH 8.0, cosubstrate 1.5 mM NADH |
696321 |
1.14.13.113 | 0.042 |
- |
Urate |
wild type enzyme, in 0.1 M potassium phosphate buffer (pH 8.0), at 25°C |
724386 |
1.14.13.113 | 0.042 |
- |
Urate |
wild-type, pH 8.0, 25°C |
724386 |
1.14.13.113 | 0.055 |
- |
NADPH |
in 50 mM K2HPO4/NaH2PO4, pH 8.0, at 25°C |
727370 |
1.14.13.113 | 0.09 |
- |
Urate |
mutant enzyme Y216F, in 0.1 M potassium phosphate buffer (pH 8.0), at 25°C |
724386 |
1.14.13.113 | 0.1 |
- |
NADH |
mutant enzyme Y216F, in 0.1 M potassium phosphate buffer (pH 8.0), at 25°C |
724386 |
1.14.13.113 | 0.1 |
- |
NADH |
mutant Y216F, pH 8.0, 25°C |
724386 |
1.14.13.113 | 0.5 |
- |
NADH |
wild type enzyme, in 0.1 M potassium phosphate buffer (pH 8.0), at 25°C |
724386 |