EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   1.1.1.244 | -999 |
- |
more |
3.8 mM and 166 mM, methanol, due to two active sites biphasic kinetics for methanol, ethanol or NAD+ in cell-free extracts, not for formaldehyde |
286372 |
| 1.1.1.244 | -999 |
- |
more |
biphasic kinetics with all alcoholic substrates and NAD+ in the presence of activator protein |
286375 |
| 1.1.1.244 | -999 |
- |
more |
Michaelis-Menten kinetics |
742514 |
| 1.1.1.244 | 0.0072 |
- |
n-butanol |
pH 9.5, 30°C, recombinant wild-type enzyme |
741715 |
| 1.1.1.244 | 0.01 |
- |
methanol |
pH 9.5, 55°C, mutant enzyme E396V |
763157 |
| 1.1.1.244 | 0.0113 |
- |
n-butanol |
pH 9.5, 30°C, recombinant mutant A31V |
741715 |
| 1.1.1.244 | 0.02 |
- |
NAD+ |
native enzyme, pH 9.5, 50°C |
656074, 656101 |
| 1.1.1.244 | 0.0216 |
- |
methanol |
pH 9.5, 30°C, recombinant mutant A26V/A31V/A169V |
741715 |
| 1.1.1.244 | 0.0269 |
- |
methanol |
pH 9.5, 30°C, recombinant mutant A169V |
741715 |
| 1.1.1.244 | 0.0307 |
- |
methanol |
pH 9.5, 30°C, recombinant mutant A26V/A169V |
741715 |
