EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.4.1.10 | -999 |
- |
more |
- |
671507 |
2.4.1.10 | -999 |
- |
more |
2.3 mM (sucrose) total, mutant enzyme S173A |
678072 |
2.4.1.10 | -999 |
- |
more |
29.2 mM (sucrose) total, mutant enzyme R370A, a 4fold KM value decrease compared with the wild-type enzyme supported the interactions of Arg 370 with the 2-OH and 3-OH of the glucosyl residue in the active site |
678072 |
2.4.1.10 | -999 |
- |
more |
31.9 mM (sucrose) total, mutant enzyme W94A, 9% of the catalytic efficiency of the wild-type |
678072 |
2.4.1.10 | -999 |
- |
more |
32 mM (sucrose), 25°C, pH 5.0, total activity |
687775 |
2.4.1.10 | -999 |
- |
more |
35.1 mM (sucrose) total, mutant enzyme N252H |
678072 |
2.4.1.10 | -999 |
- |
more |
4.1 mM (sucrose) total, mutant enzyme N252A |
678072 |
2.4.1.10 | -999 |
- |
more |
480.4 mM (sucrose) total, mutant enzyme W172A, 72fold increase of the KM of the wild-type |
678072 |
2.4.1.10 | -999 |
- |
more |
51.9 mM (sucrose) total, mutant enzyme Y421A, 3%of the wild-type activity |
678072 |
2.4.1.10 | -999 |
- |
more |
6.6 mM (sucrose) total, wild-type, pH 6.6, 37°C, 50 mM Sorensens phosphate buffer, 7.36 mg/l enzyme concentration, reaction time 60 min |
678072 |