EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    3.5.99.2 | 0.003 |
- |
thiamine |
- |
246757, 246760, 246761 |
| 3.5.99.2 | 0.058 |
- |
4-amino-5-aminomethyl-2-methylpyrimidine |
wild type enzyme, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C |
711977 |
| 3.5.99.2 | 0.068 |
- |
4-amino-5-aminomethyl-2-methylpyrimidine |
mutant enzyme F47Y, in 30 mM Tris, 50 mM NaCl (pH 8.0), at 20°C |
711977 |
| 3.5.99.2 | 0.256 |
- |
thiamine |
apparent value, in 100 mM Tris-HCl, pH 7.5, at 37°C |
713350 |
| 3.5.99.2 | 1.4 |
- |
thiamine |
wild type enzyme, at pH 7.5 and 37°C |
733003 |
| 3.5.99.2 | 2.25 |
- |
thiamine |
mutant enzyme D111A/K115A, at pH 7.5 and 37°C |
733003 |
