EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    4.1.1.7 | -999 |
- |
more |
KM-value of wild-type enzyme for 2-oxo-4-methylhexanoic acid (pH 6.0, 30°C) is above 20 mM |
666806 |
| 4.1.1.7 | 0.021 |
- |
[p-(Chloromethyl)benzoyl]formate |
- |
4799 |
| 4.1.1.7 | 0.023 |
- |
[m-(fluoromethyl)benzoyl]formate |
- |
4799 |
| 4.1.1.7 | 0.04 |
- |
benzylformate |
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C |
702341 |
| 4.1.1.7 | 0.056 |
- |
(p-methoxybenzoyl)formate |
- |
4800 |
| 4.1.1.7 | 0.059 |
- |
benzoylformate |
- |
4800 |
| 4.1.1.7 | 0.06 |
- |
benzoylformate |
mutant enzyme L461V, in potassium phosphate buffer (pH 6.5, 50 mM) |
691794 |
| 4.1.1.7 | 0.1 |
- |
2-ketopentanoate |
mutant enzyme T377L/A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C |
702341 |
| 4.1.1.7 | 0.14 |
- |
2-Oxohexanoate |
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C |
747471 |
| 4.1.1.7 | 0.14 |
- |
4-methyl-2-oxopentanoate |
mutant enzyme T377L/A460Y, at pH 6.0 and 30°C |
747471 |
