EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
5.3.1.12 | -999 |
- |
more |
primary isotope effects on the kinetic constants with D-glucuronate and the effects of changes in solvent viscosity are consistent with product release being the rate-limiting step |
702345 |
5.3.1.12 | 0.05 |
- |
D-galacturonate |
at 30°C, pH 8.0 |
690912 |
5.3.1.12 | 0.05 |
- |
D-glucuronate |
at 30°C, pH 8.0 |
690912 |
5.3.1.12 | 0.05 |
- |
D-glucuronate |
pH 8.0, 25°C, mutant H33N |
702345 |
5.3.1.12 | 0.16 |
- |
D-glucuronate |
pH 8.0, 25°C, mutant Y60F |
702345 |
5.3.1.12 | 0.2 |
- |
D-glucuronate |
pH 8.0, 25°C, mutant H33A |
702345 |
5.3.1.12 | 0.29 |
- |
D-glucuronate |
in absence of added metal or chelator |
663305 |
5.3.1.12 | 0.3 |
- |
D-glucuronate |
reconstituted with Co2+, 30°C, pH 8 |
672186 |
5.3.1.12 | 0.31 |
- |
D-glucuronate |
in presence of 0.01 mM Zn2+ |
663305 |
5.3.1.12 | 0.4 |
- |
D-glucuronate |
pH 8.0, 25°C, mutant D412A |
702345 |