EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
6.3.4.19 | -999 |
- |
more |
kinetic parameters of Escherichia coli TilS with various tRNAIle2 mutants and with tRNAMet mutants |
705718 |
6.3.4.19 | 0.00008 |
- |
precursor [tRNAIle2]-cytidine34 |
pH 7.8, 60°C |
705888 |
6.3.4.19 | 0.00014 |
- |
mature [tRNAIle2]-cytidine34 |
pH 7.8, 60°C |
705888 |
6.3.4.19 | 0.00038 |
- |
[tRNAIle2]-cytidine34 |
unmodified tRNAIle2, pH 7.8, 37°C |
705718 |
6.3.4.19 | 0.0016 |
- |
ATP |
pH 8.5, 21°C |
704642 |
6.3.4.19 | 0.0018 |
- |
7-deazaadenosine-5’-triphosphate |
pH 8.5, 21°C |
704642 |
6.3.4.19 | 0.0019 |
- |
[tRNAIle2]-cytidine34 |
pH 7.8, 60°C, mutant enzyme E106A |
706481 |
6.3.4.19 | 0.0033 |
- |
[tRNAIle2]-cytidine34 |
pH 7.8, 60°C,wild-type enzyme |
706481 |
6.3.4.19 | 0.0035 |
- |
[tRNAIle2]-cytidine34 |
pH 7.8, 60°C, mutant enzyme H133A |
706481 |
6.3.4.19 | 0.0074 |
- |
[tRNAIle2]-cytidine34 |
pH 7.8, 60°C, mutant enzyme Y114A |
706481 |