Mn2+
0.5 mM enhances the activity 7fold
Mn2+
activity dependent on
Mn2+
contains two Mn2+ ions, 0.05 mM Mn2+ used in assay conditions
Mn2+
essential for assembly of subunits
Mg2+
metalloprotein, transition metals induce aggregation and are required for catalysis, 1 equivalent of Mn2+ per subunit induces the formation of 24-mers from the trimeric enzyme
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not activated by Mg2+, Ni2+, Cd2+, Fe2+, Co2+
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not activated by Mg2+, Ni2+, Cd2+, Fe2+, Co2+, Fe3+
Mn
the manganese cluster is critical in converting the inactive trimeric state of the enzyme into its biologically active 24-mer and also forms the active site. The substrate is bound to the manganese cluster as an imidazole moiety that subsequently collapse to yield a diazafulvene intermediate
