EC Number |
Metals/Ions |
Reference |
---|
1.14.13.69 | Fe |
ICP-MS analysis of purified IsoABE is used to confirm the presence of the predicted diiron center, presence of 1.12 Fe per monomer, which is lower than the predicted 2 Fe/monomer. Purified IsoMO oxygenase is thus deficient in Fe cofactor and is expected to exhibit decreased activity as a result |
763983 |
1.14.13.69 | Iron |
alpha-helical structure that surrounds the binuclear iron binding site |
285339 |
1.14.13.69 | Iron |
binuclear non-heme iron center |
658683 |
1.14.13.69 | Iron |
enzyme contains 4 components: 1. a monomeric 35500 Da NADH reductase containing 1 mol of FAD and a probable 2Fe-2S center, 2. a 13300 Da ferredoxin containing a Rieske-type 2Fe-2S cluster, 3. a 11000 Da monomeric protein that contains no detectable cofactors, 4. a 212000 Da alpha2beta2gamma2 multimeric protein containing 4 atoms of nonheme iron. The physiological electron acceptor for the reductase is the Rieske-type ferredoxin, which is proposed to be an intermediate electron carrier between the reductase and terminal catalytic component of the system |
285342 |
1.14.13.69 | Iron |
enzyme contains a bridged dinuclear iron centre |
285337 |
1.14.13.69 | Iron |
non-haem iron |
658775 |
1.14.13.69 | Iron |
the enzyme is a non-heme diiron monooxygenase and contains a Rieske-type ferredoxin |
671530 |
1.14.13.69 | Iron |
the enzyme possesses a dinuclear iron center within the large subunit of the epoxygenase component, AmoC, mutagenesis of AmoC alters the enantioselectivity of the enzyme |
674944 |
1.14.13.69 | Iron |
the reductase component contains two prosthetic groups, an FAD centre and a [2Fe-2S] cluster. The FAD moiety is reduced by bound NADH in a two-electron reaction. The electrons are then transported to the [2Fe-2S] centre one at a time, which reduces the di-iron centre of the epoxydase. Reduction of the di-iron centre is required for oxygen binding and substrate oxidation |
285335 |
1.14.13.69 | [2Fe-2S]cluster |
the reductase component contains two prosthetic groups, an FAD centre and a [2Fe-2S] cluster. The FAD moiety is reduced by bound NADH in a two-electron reaction. The electrons are then transported to the [2Fe-2S] centre one at a time, which reduces the di-iron centre of the epoxydase. Reduction of the di-iron centre is required for oxygen binding and substrate oxidation |
285335 |