(9Z,12Z)-octadeca-9,12-dienoic acid + O2
i.e. linoleic acid
(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid + O2
i.e. alpha-linoleic acid
all-cis-9,12,15-octadecatrienoic acid + O2
i.e. alpha-linoleic acid
all-cis-9,12-octadecadienoic acid + O2
i.e. linoleic acid
more
both the wild type ZmLOX and A562G mutant dioxygenate monolinolenoylglycerol and 2-linoleoyl-sn-glycero-3-phosphorylcholine, the latter being a poor substrate. Both oxidize the monolinolenoylglycerol predominantly into (9S)-hydroperoxide. The oxidation of 2-linoleoyl-sn-glycero-3-phosphorylcholine exhibits limited regio- and stereospecificity: the wild-type ZmLOX produces some predominance of (13S)-hydroperoxide. In contrast, the A562G mutant produces some excess of (9S)-hydroperoxide
