oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-16beta
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-16beta, main activity of enzyme CYP716A141
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-28
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-28, reaction of EC 1.14.13.201
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products