methylenetetrahydrofolate dehydrogenase (NADP+)
5,10-methylenetetrahydrofolate + NADP+
-
5,10-methenyltetrahydrofolate + NADPH + H+
-
formyltetrahydrofolate dehydrogenase
10-formyltetrahydrofolate + NADP+ + H2O
-
tetrahydrofolate + CO2 + NADPH + H+
-
formyltetrahydrofolate dehydrogenase
10-formyltetrahydrofolate + NADP+ + H2O
natural substrate
tetrahydrofolate + CO2 + NADPH + H+
-
formyltetrahydrofolate dehydrogenase
10-formyltetrahydrofolate + NADP+ + H2O
recycling of excess 10-formyltetrahydrofolate that is not needed for purine biosynthesis and restoration of the tetrahydrofolate pool, important for formate metabolism by clearing it as CO2 and thus protecting cells from formate intoxication
tetrahydrofolate + CO2 + NADPH + H+
-
formyltetrahydrofolate dehydrogenase
10-formyltetrahydrofolate + NADP+ + H2O
regulatory mechanism to control the in vivo folate pool size
tetrahydrofolate + CO2 + NADPH + H+
-
formyltetrahydrofolate dehydrogenase
10-formyltetrahydrofolate + NADP+ + H2O
disposal of excess one-carbon units, oxidation of one-carbon moieties is regulated by the ratio of formyltetrahydrofolate to tetrahydrofolate in liver, regulation of the proportion of folate present in the tetrahydrofolate form, presumably to make it available for other reactions of one-carbon metabolism
tetrahydrofolate + CO2 + NADPH + H+
-
formyltetrahydrofolate dehydrogenase
10-formyltetrahydrofolate + NADP+ + H2O
folate-dependent metabolism of formate to carbon dioxide
tetrahydrofolate + CO2 + NADPH + H+
-
formyltetrahydrofolate dehydrogenase
10-formyltetrahydrofolate + NADP+ + H2O
a major folate-binding protein of liver cytosol, 10-formyltetrahydrofolate polyglutamates are tightly bound in vivo
tetrahydrofolate + CO2 + NADPH + H+
-
formyltetrahydrofolate dehydrogenase
10-formyltetrahydropteroylpentaglutamate + NADP+ + H2O
-
tetrahydropteroylpentaglutamate + CO2 + NADPH
-
formyltetrahydrofolate dehydrogenase
10-formyltetrahydropteroylpentaglutamate + NADP+ + H2O
natural substrate
tetrahydropteroylpentaglutamate + CO2 + NADPH
-
