EC Number   |
Natural Substrates |
|---|
   1.13.12.16 | 3 propionate-3-nitronate + O2 |
- |
| 1.13.12.16 | ethylnitronate + O2 |
- |
| 1.13.12.16 | ethylnitronate + O2 + FMNH2 |
- |
| 1.13.12.16 | more |
active on primary and secondary nitroalkanes, with a marked preference for unbranched primary nitroalkanes |
| 1.13.12.16 | more |
enzyme catalyzes the oxygenative denitrification of anionic nitroalkanes much more effectively than that of the neutral ones |
| 1.13.12.16 | more |
enzyme is more specific for nitronates than nitroalkanes |
| 1.13.12.16 | more |
both the neutral and anionic forms of nitroalkanes act as substrates for the oxidative denitrification reaction catalyzed by Neurospora crassa NMO. NMO does not produce and release hydrogen peroxide during turnover with linear alkyl nitronates of various lengths between 2 and 6 carbon atoms or with propyl-2-nitronate. With the exception of propyl-1- and propyl-2-nitronate, there is no release of superoxide during turnover of NMO at pH 8.0 and 30°C with linear alkyl nitronates with chain lengths between 2 and 6 carbon atoms |
| 1.13.12.16 | more |
only alkyl nitronates are used as substrates in the oxidative denitrification reaction catalyzed by Williopsis saturnus var. mrakii NMO, nitroalkanes are no substrates. The different substrate specificity compared to other NMOs might result from the presence of a His residue in the active site and conformational differences. NMO does not produce and release hydrogen peroxide during turnover with linear alkyl nitronates of various lengths between 2 and 6 carbon atoms or with propyl-2-nitronate. With the exception of propyl-2-nitronate, there is no release of superoxide during turnover of NMO at pH 8.0 and 30°C with linear alkyl nitronates with chain lengths between 2 and 6 carbon atoms |
| 1.13.12.16 | nitroethane + O2 |
- |
| 1.13.12.16 | propionate 3-nitronate + O2 |
- |
