EC Number   |
Natural Substrates |
|---|
    4.1.99.22 | (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate |
reaction of MoaC |
| 4.1.99.22 | GTP |
- |
| 4.1.99.22 | GTP |
the enzyme catalyses an early step in the biosynthesis of the molybdenum cofactor |
| 4.1.99.22 | GTP |
MoaA cleaves GTP by a radical mechanism, a 5'-desoxyadenosyl radical is generated from S-adenosyl-L-methionine at the N-terminal cluster facilitating hydrogen abstraction at either the C8 of the guanine or the C2' or C3' atoms of the ribose. Insertion of the formyl group between the ribose C2' and C3' carbons might also require radical mediation. MoaC is involved in the cleavage of the dihydropyrazine-type intermediate pyrophosphate group and formation of the cPMP cyclic phosphate group |
| 4.1.99.22 | GTP |
reaction of MoaA with GTP, S-adenosyl-L-methionine, and sodium dithionite in the absence of MoaC |
| 4.1.99.22 | GTP + S-adenosyl-L-methionine + reduced electron acceptor |
- |
| 4.1.99.22 | more |
MoaA catalyzes a unique radical C-C bond formation reaction via a 5'-deoxyadenosyl radical intermediate and that, in contrast to previous proposals, MoaC plays a major role in the complex rearrangement to generate the pyranopterin ring |
