Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Application

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term: analysis

<< < Results 1301 - 1400 of 1617 > >>
EC Number Recommended Name Application Commentary
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.23matrilysin analysis method to localize enzyme activity within tissues by selective degradation of crosslinked carboxymethylated transferrin on polyethylene films
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.23matrilysin analysis use of substrate PB-M7vis as a proteolytic beacon and optical molecular imaging contrast reagent for in vivo detection of enzyme activity
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.27thermolysin analysis thermolysin degrades cellular prion protein while preserving both proteinase K-sensitive and proteinase K-resistant isoforms of disease-related prion protein in both rodent and human prion strains. In variant Creutzfeldt-Jakob disease, up to 90% of total prion protein present in the brain resists degradation with thermolysin, whereas only about 15% of this material resists digestion by proteinase K
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.27thermolysin analysis selective biotin tagging and thermolysin proteolysis of chloroplast outer envelope proteins reveals information on protein topology and association into complexes. Development and evaluation of a method providing information at the surface of the outer envelope membrane, based on specific tagging with biotin or proteolysis using thermolysin, a non-membrane permeable protease. Envelope, thylakoid, and stroma proteins are separated by two-dimensional electrophoresis and analyzed by immunostaining and mass spectrometry, overview
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.27thermolysin analysis thermolysin offers a tool for complete solubilization of cartilage prior to comprehensive glycosaminoglycans(GAG)omic analysis, and is likely applicable to other collagen-rich tissues such as ligaments, skin, and blood vessels
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.33peptidyl-Asp metalloendopeptidase analysis analysis of isoaspartic acid by selective proteolysis with Asp-N and electron transfer dissociation mass spectrometry, overview. IsoAsp formation and repair is central to the survival and germination of plant seeds. Also once administered into patients and thus exposed to physiological conditions of pH 7 and 37 °C, protein pharmaceuticals, particularly those with long circulation time, may generate significant amount of isoAsp
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.56insulysin analysis immunocapture-based assay that uses the fluorogenic peptide substrate (7-methoxycoumarin-4-yl)acetyl-RPPGFSAFK-2,4-dinitrophenyl and allows the specific measurement of insulin-degrading enzyme activity in brain tissue homogenates. The fluorogenic substrate can be cleaved by a number of enzymes including neprilysin endothelin-converting enzyme-1 and angiotensin-converting enzyme, as well as IDE. Discrimination between these individual enzymes is not readily achieved in tissue homogenates, even in the presence of selective inhibitors and pH conditions. Immunocapture with antibody to the inactive domain of IDE prior to the addition of fluorogenic substrate allows sensitive, linear at 156-2500 ng/ml, and specific measurement of IDE activity and negligible cross-reactivity with neprilysin, endothelin-converting enzyme-1 or angiotensin-converting enzyme
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.65macrophage elastase analysis the enzyme can be utilized for pharmacological evaluation of anti-inflammatory mechanisms of action
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.67choriolysin H analysis developmental expression patterns of the Chrysiptera parasema HCE gene are highly similar to that of the medaka HCE gene, hatching enzyme system is highly conserved between marine and freshwater fish species
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.67choriolysin H analysis the eel enzymes resemble the high choriolytic enzyme of medaka Oryzias latipes, and the hatching enzymes of the zebra fish Danio rerio and masu salomon Oncorhynchus masou, early differentation profile of eel hatching gland cells is similar to that of medaka, masu salomon and zebra fish, whereas the final location of the gland cells was different among fishes
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.69bontoxilysin analysis development and evaluation of molecular detection tool for tracking and tracing Clostridium botulinum types A, B, E, F and other botulinum neurotoxin producing Clostridia using the real-time PCR-based GeneDisc cycler and BoNTs, overview. No cross reactivity with non human-toxigenic bacteria, Clostridium botulinum types C, D and G
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.69bontoxilysin analysis the recombinant VAMP2 peptide substrate can be used as in vitro bioassay for the detection of BONT/B in food samples and provide a relevant replacement assay for the estimation of the toxin potency in contaminated therapeutic preparations
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.75lysostaphin analysis improvement of lipid extraction of staphylococcal cells
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.80membrane-type matrix metalloproteinase-1 analysis screening, identification, and characterization of affinity peptides, AF7p and Cy5.5-HS7, specific to MT1-MMP and application in tumor imaging, overview. Feasibility of using the subtraction biopanning strategy to screen the affinity peptide targeting MT-loop regions and HS7 is a superior probe for noninvasively imaging MT1-MMP expression in MT1-MMP-positive tumor models. Usage of HS7 in early diagnosis of tumors and in peptide-mediated drugs
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.86ADAM 17 endopeptidase analysis development of a fluorescence resonance energy transfer-based biosensor that quantitatively reports the kinetics of TACE activity in live cells
Display the word mapDisplay the reaction diagram Show all sequences 3.4.24.87ADAMTS13 endopeptidase analysis development of a 77-amino acid flow cytometry substrate to measure ADAMTS13 activity. Substrate is suitable for microsphere-cytometer based assays in the milieu of human blood plasma. It contains the partial A2-domain of von Willebrand factor (VWF amino acids 1594-1670) that is mutated to include a single primary amine at the N-terminus and free cysteines at the C-terminus. Substrate is bound to streptavidin-bearing microspheres with varyable polyethylene glycol spacer lengths. Recombinant ADAMTS13 activity can be quantified using substrates with all varyable polyethylene glycol repeat-lengths, but only a construct with the longer 77 PEG-unit can quantify proteolysis in blood plasma. Plasma ADAMTS13 down to 5% of normal levels can be detected within 30 min. Enzyme catalytic activity is tuned by varying buffer calcium, with lower divalent ion concentrations enhancing cleavage
Display the word mapDisplay the reaction diagram Show all sequences 3.4.25.1proteasome endopeptidase complex analysis simultaneous labeling and detection of constitutive 20S proteasome subunits using an affinity-based probe cocktail and application to biological samples
Display the word mapDisplay the reaction diagram Show all sequences 3.4.99.B2D-aspartyl endopeptidase analysis a system for screening D-Asp-containing proteins is developed by using D-aspartyl endopeptidase and a two-dimensional gel electrophoresis
Show all pathways known for 3.5.1.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.1asparaginase analysis direct measurement of L-asparagine in human plasma samples through the use of Escherichia coli Lasparaginase in the soluble form is a major clinical application of this system
Show all pathways known for 3.5.1.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.1asparaginase analysis biosensor for asparagine using a thermostable recombinant asparaginase from Archaeoglobus fulgidus immobilized in front of an ammonium-selective electrode. The biosensor has a detection limit of 0.06 mM for L-asparagine. It shows high stability
Show all pathways known for 3.5.1.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.1asparaginase analysis assembly a microplate-based biosensor for the determination of L-asparagine in biological samples. The enzyme is immobilized by crosslinking with glutaraldehyde in a microplate in 96-well format. The sensing is based on the colorimetric measurement of ammonia formation using the Nessler's reagent. The sensor enables monitoring of L-asparagine levels in serum and foods samples in the concentration range 10-200 microM, with a detection limit of 10 microM for L-asparagine
Show all pathways known for 3.5.1.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.1asparaginase analysis method based on IR spectroscopy to determine PEG-chitosan copolymer composition as well as composition of copolymer-enzyme conjugates. The method is reagentx02free and allows fast and reliable determination of parameters
Show all pathways known for 3.5.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.2glutaminase analysis construction of a L-glutaminase enzyme reactor based on immobilization of enzyme onto bamboo sticks through a glutaraldehyde modification to achieve covalent bonding. L-glutaminase-bamboo exhibits improved enzymatic hydrolysis performances, including high hydrolysis efficiency, prolonged stability (14 days) and good reusability and can efficiently be uesd for inhibitor screening
Show all pathways known for 3.5.1.3Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.3omega-amidase analysis use of soluble omega-amidase-glutamate dehydrogenase to determine alpha-ketoglutaramate in biological samples
Show all pathways known for 3.5.1.5Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.5urease analysis urea is estimated in different blood samples with the help of agar-immobilized urease and the results are consistent with those from clinical pathology laboratory through an autoanalyzer
Show all pathways known for 3.5.1.5Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.5urease analysis urease is used to calculate the amount of urea in biological solutions in order to remove urea from blood, waste water, fruit juice and foods. Enhancement of stability of immobilised urease by biocompatible polymer-conjugated magnetic beads for industrial application based on removal of urea
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.12biotinidase analysis identification of the site of carboxylation of the biotinyl prosthetic group of several biotin enzymes
Show all pathways known for 3.5.1.14Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.14N-acyl-aliphatic-L-amino acid amidohydrolase analysis enzyme widely used as reagent to resolve amino acid racemates
Show all pathways known for 3.5.1.19Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.19nicotinamidase analysis determination of nicotinamide
Show all pathways known for 3.5.1.19Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.19nicotinamidase analysis nicotinamidases are key analytical biocatalysts for screening modulators in relevant biomedical enzymes, such as sirtuins and poly-ADP-ribosyltransferases
Show all pathways known for 3.5.1.23Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.23ceramidase analysis development of a method for the specific visualization of catalytically active acid ceramidase in intracellular compartments is crucial for diagnosis and follow-up of therapeutic strategies in diseases linked to altered enzyme activity
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.32hippurate hydrolase analysis enzymatic determination of Bacillus subtilis siderophore itoic acid and 2,3-dihydroxybenzoic acid
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.41chitin deacetylase analysis the enzyme can be used for production of chitin particles with only 1% deacetylated chitin which is still rigid and insoluble in acidic environment, but shows highly increased ovalbumin binding, chitosan as well is a good material for column chromatography showing no hydrogel formation
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.52peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase analysis valuable tool to characterize the peptide portion of glycoproteins and glycopeptides to release asparagine-linked oligosaccarides for structural analysis, recombinant PNGase F contains no contaminant Endo F, proteases or exoglycosidases
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.52peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase analysis a procedure to map N-glycosylation sites is presented, it can be applied to purified proteins as well as to highly complex mixtures. The method exploits deglycosylation by PNGase F in a diagonal, reverse-phase chromatographic setup
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.52peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase analysis the facile expression, non-glycosylated nature, unusual pH optimum and broad substrate specificity of the enzyme makes recombinant PNGase H+ a versatile tool in N-glycan analysis
Display the reaction diagram Show all sequences 3.5.1.69glycosphingolipid deacylase analysis the enzyme is immobilised on magnetic macroporous cellulose and is used to semisynthesise C17:0 glucosylceramide and C17:0 sulfatide, which are required as internal standards for quantification of the corresponding glycosphingolipids by tandem mass spectrometry
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.75urethanase analysis the enzyme is used in spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactions, method, overview. Detection of ethyl carbamate (urethane) in Chinese rice wine. Urethane is known as a genotoxic carcinogen1 that widely exists in fermented foods and alcoholic beverages, such as bread, yogurt, cheese, brandy, Chinese rice wine, sake, and wine, due to the natural biochemical processes in the fermentation process
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.88peptide deformylase analysis novel class of N-formylated peptides for routine kinetic characterization and for screening PDF inhibitors
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.103N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase analysis fluorescence-based assay for measuring N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase activity that does not require HPLC analysis and can be carried out in multiwell plates. The fluorescamine-based assay can be used to determine the steady-state parameters for the deacetylation of N-acetyl-glucosamine by MshB
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.119Pup amidohydrolase analysis development of an in vitro activity assay for Dop, based on fluorescence anisotropy measurements. The assay is simple, sensitive, and compatible with a high-throughput format for screening purposes. It can be reliably and conveniently used for detailed kinetic measurements of Dop activity and can be employed with other Dop enzymes
Show all pathways known for 3.5.2.10Display the word mapDisplay the reaction diagram Show all sequences 3.5.2.10creatininase analysis analysis of serum creatinine concentration
Show all pathways known for 3.5.2.10Display the word mapDisplay the reaction diagram Show all sequences 3.5.2.10creatininase analysis construction of a biosensor by co-immobilization of creatininase, creatinase, and sarcosine oxidase onto iron oxide nanoparticles/chitosangraft-polyaniline, Fe3O4-NPs/CHIT-g-PANI, composite film electrodeposited on surface of Pt electrode through glutaraldehyde coupling. The creatinine biosensor uses enzymes/Fe3O4-NPs/CHIT-g-PANI/Pt electrode as working electrode, Ag/AgCl as reference electrode and Pt wire as auxiliary electrode. The biosensor exhibits an optimum response within 2 s at pH 7.5 and 30°C, when polarized at 0.4 V versus Ag/AgCl. The electrocatalytic response shows a linear dependence on creatinine concentration ranging from 1 to 800 microM. The sensitivity of the biosensor is 3.9 microA per microM and cm2, with a detection limit of 1 microM. The biosensor shows only 10% loss in its initial response after 120 uses over 200 days, when stored at 4°C. The biosensor measures creatinine in the serum of apparently healthy persons
Show all pathways known for 3.5.2.14Display the word mapDisplay the reaction diagram Show all sequences 3.5.2.14N-methylhydantoinase (ATP-hydrolysing) analysis measurement of serum and urine creatinine
Display the reaction diagram Show all sequences 3.5.2.20isatin hydrolase analysis a fluorescence-based enzymatic assay is developed that can quantify isatin, a putative stress biomarkerin blood samples. A phase extraction of isatin followed by a second phase extraction combined with an enzymatic reaction performed by an isatin hydrolase is used to extract and quantify isatin in whole blood samples
Show all pathways known for 3.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.1arginase analysis specific detection of NO production in intact mouse tissue, inhibition of enzyme by N-hydroxy-nor-L-arginine to avoid disturbances
Show all pathways known for 3.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.1arginase analysis arginase activity may interfere in nitric oxide activity assay. A nitric oxide synthase-independent radioactive signal in mitochondrial samples analyzed for nitric oxide synthase-catalyzed [14C]-L-arginine to [14C]-L-citrulline conversion is due to the arginase-catalyzed conversion of [14C]-L-arginine to [14C]-urea. The results, in addition to reconfirming the absence of nitric oxide synthase activity in rat liver MT, show the need to include arginase inhibitors in studies using mitochondrial samples in order to avoid confounding results when using nitric oxide synthase activity assays
Show all pathways known for 3.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.1arginase analysis assay method based on a combination of moderately selective host-guest binding with the specificity of enzymatic transformations which allows the real-time monitoring of enzymatic reactions in a homogeneous solution. The resulting supramolecular tandem assays exploit the dynamic binding of a fluorescent dye with a macrocyclic host in competition with the binding of the substrate and product. The depletion of the substrate allows the fluorescent dye to enter the macrocycle in the course of the enzymatic reaction, which leads to the desired fluorescence response. For arginase, p-sulfonatocalix[4]arene is used as the macrocycle, which displays binding constants of 6400 per M with arginine, 550 per M with ornithine, and 60 000 per M with the selected fluorescent dye 1-aminomethyl-2,3-diazabicyclo[2.2.2]oct-2-ene, the dye shows a weaker fluorescence in its complexed state, which leads to a switch-off fluorescence response in the course of the enzymatic reaction. Assays can be successfully used to probe the inhibition of enzymes
Show all pathways known for 3.5.3.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.1arginase analysis development of a high-throughput semiquantitative assay system using a colorimetric 96-well plate assay to monitor the formation of urea. The assay has a dynamic range of about 5-300 microM for the ureido product
Show all pathways known for 3.5.3.3Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.3creatinase analysis biomatrix fabricated to investigate immobilization of creatine amidinohydrolase
Show all pathways known for 3.5.3.3Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.3creatinase analysis construction of a biosensor by co-immobilization of creatininase, creatinase, and sarcosine oxidase onto iron oxide nanoparticles/chitosangraft-polyaniline, Fe3O4-NPs/CHIT-g-PANI, composite film electrodeposited on surface of Pt electrode through glutaraldehyde coupling. The creatinine biosensor uses enzymes/Fe3O4-NPs/CHIT-g-PANI/Pt electrode as working electrode, Ag/AgCl as reference electrode and Pt wire as auxiliary electrode. The biosensor exhibits an optimum response within 2 s at pH 7.5 and 30°C, when polarized at 0.4 V versus Ag/AgCl. The electrocatalytic response shows a linear dependence on creatinine concentration ranging from 1 to 800 M. The sensitivity of the biosensor is 3.9 microA per microM and cm2, with a detection limit of 1 microM. The biosensor shows only 10% loss in its initial response after 120 uses over 200 days, when stored at 4°C. The biosensor measures creatinine in the serum of apparently healthy persons
Show all pathways known for 3.5.3.9Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.9allantoate deiminase analysis development of an enzyme cycling method for measuring allantoin concentrations in human serum
Show all pathways known for 3.5.3.12Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.12agmatine deiminase analysis development of a multiplex PCR method for the simultaneous detection of four genes involved in the production of histamine, i.e. histidine decarboxylase hdc, tyramine, i.e.tyrosine decarboxylase tyrdc, and putrescine, via either ornithine decarboxylase odc, or agmatine deiminase agdi. A collection of 810 lactic acid bacteria strains isolated from wine and cider was screened. The most frequent gene corresponds to the agdi gene detected in 112 strains, 14% of all lactic acid bacteria strains, of 10 different lactic acid bacteria species
Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.15protein-arginine deiminase analysis adding bicarbonate to commercial peptidyl arginine deiminase activity kits could increase assay performance and biological relevance
Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.15protein-arginine deiminase analysis development of a fluorescent probe to detect citrulline with high sensitivity and broad dynamic range and establishment of multiple assay systems for PAD subtypes activities, including PAD2 and PAD4, with the probe
Display the word mapDisplay the reaction diagram Show all sequences 3.5.3.18dimethylargininase analysis use of inhibitor N-(but-3-yn-1-yl)-2-chloroethanimidamide as a broad-specificity probe for labeling endogenous DDAH isoforms and enzymes with similar pharmacophores. Inhibitor labels the active fraction of DDAH-1 in intact mammalian cells and can be blocked by the presence of competitive reversible and irreversible inhibitors. Incorporation of the alkyne tag allows to derivatize with a variety of reagents after in vivo tagging
Show all pathways known for 3.5.4.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.1cytosine deaminase analysis crystallization strategy named microseed matrix screening, differential chelation pattern of cations by acidic surfaces of proteins within crystal lattice as a critical parameter of crystal nucleation and growth
Show all pathways known for 3.5.4.1Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.1cytosine deaminase analysis in a selection-rooting assay CodA-expressing aerial tissues or shoot cuttings of Glycine max are inhibited for root formation in media containing 5-fluorouracil
Show all pathways known for 3.5.4.3Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.3guanine deaminase analysis colorimetric and HPLC assay method using guanosine as a prosubstrate. Method is suitable for routine assays for measuring plasma enzyme over a wide range of activites
Show all pathways known for 3.5.4.4Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.4adenosine deaminase analysis a capillary electrophoresis method for simultaneous analysis of adenosine deaminase in red blood cells is developed. The method is also successfully applied in the inhibitor screening from traditional Chinese medicines
Show all pathways known for 3.5.4.4Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.4adenosine deaminase analysis analysis of adenosine deaminase in saliva could be used as a simple, rapid, economic and non-invasive diagnostic tool in porcine production in field conditions
Show all pathways known for 3.5.4.6Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.6AMP deaminase analysis assessment of synthetic nitro- and polycyclic musks, imidazolium ionic liquids and N-glucopyranosyl ammonium salts
Show all pathways known for 3.5.4.21Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.21creatinine deaminase analysis analysis of creatinine in serum and urine
Show all pathways known for 3.5.4.21Display the word mapDisplay the reaction diagram Show all sequences 3.5.4.21creatinine deaminase analysis the use of an ammonium ion-selective composite material, based on polyaniline and copper is reported for use as a transducer in electrochemical hydrolase-based urea and creatinine biosensors. The experimental designs and analytical performance of the biosensors are detailed together with their application for the determination of creatinine and urea in serum samples from patients with chronic kidney disease. A combination of creatinine deaminase and urease is chosen as a model system to demonstrate the construction of urea and creatinine biosensors to illustrate the principle
Display the word mapDisplay the reaction diagram Show all sequences 3.5.5.1nitrilase analysis tools in the biodegradation and biodetection of cyanide
Display the word mapDisplay the reaction diagram Show all sequences 3.6.1.2trimetaphosphatase analysis histochemical marker for lysosomes in light and electron microscopy
Show all pathways known for 3.6.1.5Display the word mapDisplay the reaction diagram Show all sequences 3.6.1.5apyrase analysis immune sera that recognize specifically the B domain of NTPDase 1 are produced against synthetic peptides (LbB1LJ and LbB2LJ) derived from this domain of NTPDase from Leishmania brasiliensis. The polyclonal antibodies have effective anti-leishmanial effect, reducing significantly in vitro promastigotes growth (21-25%), an antiproliferative effect is also demonstrated by immune sera produced against recombinant r-pot B domain, and two other synthetic peptides (potB1LJ and potB2LJ). In addition, using these biomolecules in ELISA technique, IgG1 and IgG2 subclasses reactivities of either healthy dogs or infected by Leishmania infantum and classified clinically as asymptomatic, oligosymptomatic and symptomatic are tested. Analysis of distinct IgG1 and IgG2 seropositivities patterns suggest antibody subclasses binding epitopes along B domain for protection against infection, indicating this domain as a tool for prophylactic and immunotherapeutic investigations
Show all pathways known for 3.6.1.5Display the word mapDisplay the reaction diagram Show all sequences 3.6.1.5apyrase analysis usage of the enzyme in a two-dimensional array ATP/ADP sensitive image sensor, with a uniform distribution of chemically immobilized apyrase (via two different methods (3-APTES and CEST)). The enzyme sensor changes ATP into AMP, and the concentration of ATP reduces with time. The time-dependent profile of the potential response is different from the common ion sensor on which the concentration of the analyte ion is constant at an equilibrium state. Two ways of the measurement are used: 1. the addition measurement and 2. the steady measurement, overview
Display the word mapDisplay the reaction diagram Show all sequences 3.6.1.6nucleoside diphosphate phosphatase analysis because of its resistance against lipid peroxidation nucleoside diphosphatase is a well-suited intrinsic parameter to estimate this effect of lipid peroxidation on the microsomal membrane
Display the word mapDisplay the reaction diagram Show all sequences 3.6.1.10endopolyphosphatase analysis the overexpression of the processed form of the enzyme should provide a unique and powerful reagent to analyze inorganic polyphosphate when the chain termini are unavailable to the actions of polyPase and polyP kinase
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B1kinesin K16 analysis photoreactiveATP derivative, 2'(3')-O-(4-benzoylbenzoyl)-1,N6-etheno-ATP acts as a cross-linker to detect conformational changes in the kinesin motor domain
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.B7RadA recombinase analysis the highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances the specificity of simplex and multiplex PCR assays
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.10non-chaperonin molecular chaperone ATPase analysis high-throughput screening method in 96-well plates for ATPase activity of DnaK. Screening of potential inhibitors
Display the word mapDisplay the reaction diagram Show all sequences 3.6.5.2small monomeric GTPase analysis nucleotide exchange kinetics with small GTPases can be used to obtain important information about the binding kinetics of GTP molecules under different activation states and with different activator molecules
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2(S)-2-haloacid dehalogenase analysis the enzyme is covalently linked to an N-hydroxysuccinimidyl Sepharose resin to construct a highly specific sensor with long shelf life for the detection of L-2-haloacids
Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.3haloacetate dehalogenase analysis selection marker on cloning vectors
Show all pathways known for 3.8.1.8Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.8atrazine chlorohydrolase analysis fiber-optic biosensors for detection of atrazine at low concentrations in soil using the atrazine chlorohydrolase and quantification of hydrochloric acid release, optimization, overview
Display the reaction diagram Show all sequences 3.9.1.2protein arginine phosphatase analysis development of a substrate-trapping mutant that retains binding affinity toward arginine-phosphorylated proteins but cannot hydrolyze the captured substrates. Mutant C9A stably binds to arginine-phosphorylated proteins. The substrate-trapping efficiency is improved by impeding the oligomerization of the phosphatase mutant. The engineered YwlE trap efficiently captures arginine-phosphorylated proteins from complex Bacillus subtilis ywlE- cell extracts, thus facilitating identification of phosphoarginine sites in the large pool of cellular protein modifications
Display the word mapDisplay the reaction diagram Show all sequences 3.11.1.2phosphonoacetate hydrolase analysis activity stain for detection of carbon-phosphorus cleavage by phosphonoacetate hydrolase in PAGE gels
Display the word mapDisplay the reaction diagram Show all sequences 3.12.1.B1tetrathionate hydrolase analysis measurement of enzyme activity is based on a continuous spectrophotometric method that detects soluble intermediates that absorb in the UV region
Display the word mapDisplay the reaction diagram Show all sequences 3.12.1.B1tetrathionate hydrolase analysis measurement of the enzyme activity during purification is based on the absorbance of the initial intermediates formed from tetrathionate in the ultraviolet region. Enzyme activity can also be measured by the scattering of insoluble sulfur in the visible region
Show all pathways known for 3.13.2.1Display the reaction diagram Show all sequences 3.13.2.1adenosylhomocysteinase analysis study of methylation reactions
Show all pathways known for 3.13.2.1Display the reaction diagram Show all sequences 3.13.2.1adenosylhomocysteinase analysis simple colorimetric method for the sensitive detection of S-adenosylhomocysteine hydrolase activity and inhibition using fluorosurfactant-capped gold nanoparticles. After hydrolysis of S-adenosylhomocysteine, the produced homocysteine molecules are bound to the surface of Au nanoparticles through the formation of Au-S bonds. The approach has a minimum detectable concentration of 100 units/l, or about 6 nM. The addition of adenosine analogs to a solution containing S-adenosylhomocysteine and S-adenosylhomocysteine hydrolase results in the suppression of hydrolyzed S-adenosylhomocysteine-induced nanoparticle aggregation
Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.4acetoacetate decarboxylase analysis the enzyme is a valuable tool for clinical analysis of ketone bodies in human plasma
Show all pathways known for 4.1.1.15Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.15glutamate decarboxylase analysis most important application of glutamate decarboxylase antibodies is to identify those neurons and neuronal projections that use 4-aminobutanoate as their neurotransmitter by immunocytochemical visualization
Show all pathways known for 4.1.1.15Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.15glutamate decarboxylase analysis the diabetes autoantibody standardization program, DASP, aims to improve and standardize measurement of autoantibodies, e.g. glutamic acid decarboxylase autoantibodies and islet antigen-2, against associated with type 1 diabetes, overview
Show all pathways known for 4.1.1.21Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.21phosphoribosylaminoimidazole carboxylase analysis sensitive and rapid analytical method for detection of purine de novo biosynthesis intermediates based on high performance liquid chromatography with electrochemical detection. Method is applied to accumulation of 5-aminoimidazole ribotide in AdeD cells
Show all pathways known for 4.1.1.28Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.28aromatic-L-amino-acid decarboxylase analysis development o a high-throughput assay for testing of inhibitors
Show all pathways known for 4.1.1.28Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.28aromatic-L-amino-acid decarboxylase analysis loss of dopa decarboxylase appears to result in similar phenotypes as those of aromatic L-amino acid decarboxylase deficiency, thus zebrafish could be a good model for investigating pathogenetic mechanisms of aromatic L-amino acid decarboxylase deficiency in children
Show all pathways known for 4.1.1.39Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.39ribulose-bisphosphate carboxylase analysis 1 pg of plant mRNA, ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, is used to determine the efficiency of RT-PCR in general, and as a external reference gene for normalization of gene expression
Show all pathways known for 4.1.1.39Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.39ribulose-bisphosphate carboxylase analysis a method is established to remove ribulose bisphosphate carboxylase/oxygenase from plant samples to obtain high quality and high resolution 2D gels in proteome analysis
Show all pathways known for 4.1.1.39Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.39ribulose-bisphosphate carboxylase analysis a sensitive and robust mixed-mode high performance liquid chromatography-tandem mass spectrometry method is developed for the qualitative and quantitative determination of sugar phosphates
Show all pathways known for 4.1.1.49Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.49phosphoenolpyruvate carboxykinase (ATP) analysis bicarbonate assay
Show all pathways known for 4.1.1.50Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.50adenosylmethionine decarboxylase analysis development of a simple, economic, and non-radioactive spectrometric enzymatic assay, which can be adapted for experimental high-throughput screening of AdoMetDC inhibitors
Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.53phenylalanine decarboxylase analysis use of enzyme for decarboxylation of phenylalanine to increase its volatility for continuous-flow isotopic analysis without introducing extraneous C or significant isotopic fractionation
Show all pathways known for 4.1.1.65Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.65phosphatidylserine decarboxylase analysis development of a fluorescence-based assay for selectively monitoring production of phosphatidylethanolamine in reactions using a maltose-binding protein fusion with Plasmodium knowlesi PSD as the enzyme. The product detection by fluorescence occurs after the lipid reacts with a water-soluble distyrylbenzene-bis-aldehyde and provides strong discrimination against the phosphatidylserine substrate. The assay is amenable to application in 96- and 384-well microtiter plates
Show all pathways known for 4.1.1.65Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.65phosphatidylserine decarboxylase analysis use of the complementation of a Saccharomyces cerevisiae mutant for screening inhibitors
Show all pathways known for 4.1.2.20Display the word mapDisplay the reaction diagram Show all sequences 4.1.2.202-dehydro-3-deoxyglucarate aldolase analysis enzymatic determination of D-glucaric acid
Show all pathways known for 4.1.2.20Display the word mapDisplay the reaction diagram Show all sequences 4.1.2.202-dehydro-3-deoxyglucarate aldolase analysis specific enzymatic assay for D-glucarate in human serum
Display the word mapDisplay the reaction diagram Show all sequences 4.1.2.22fructose-6-phosphate phosphoketolase analysis the gene encoding xylulose-5-phosphate/fructose-6-phosphate phosphoketolase (xfp) is conserved among Bifidobacterium species within a more variable region of the genome, useful for strain identification
<< < Results 1301 - 1400 of 1617 > >>