EC Number   |
Recommended Name |
Application |
|---|
   3.1.8.2 | diisopropyl-fluorophosphatase |
degradation |
- |
 1.97.1.1 | chlorate reductase |
degradation |
(per)chlorate-reducing microorganisms are useful for bioremediation of soils and sediments |
| 3.2.1.21 | beta-glucosidase |
degradation |
a 3.43fold synergistic effect by combining with Trichoderma reesei cellulases is observed |
| 3.1.1.101 | poly(ethylene terephthalate) hydrolase |
degradation |
a dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films. Since the enzymatic PET hydrolysis is inhibited by the degradation intermediate 4-[(2-hydroxyethoxy)carbonyl]benzoate, a dual enzyme system consisting of a polyester hydrolase and the immobilized carboxylesterase TfCa from Thermobifida fusca KW3 is employed for the hydrolysis of PET films at 60°C. HPLC analysis of the reaction products obtained after 24 h of hydrolysis shows an increased amount of soluble products with a lower proportion of 4-[(2-hydroxyethoxy)carbonyl]benzoate in the presence of the immobilized carboxylesterase TfCa. The results indicate a continuous hydrolysis of the inhibitory 4-[(2-hydroxyethoxy)carbonyl]benzoate by the immobilized carboxylesterase TfCa and demonstrate its advantage as a second biocatalyst in combination with a polyester hydrolase for an efficient degradation oft PET films |
| 3.1.1.101 | poly(ethylene terephthalate) hydrolase |
degradation |
a dual enzyme system consisting of the polyester hydrolase and the immobilized carboxylesterase TfCa from Thermobifida fusca KW3 can be employed for the hydrolysis of PET films at 60°C, resulting in an increased amount of soluble products with a lower proportion of mono-(2-hydroxyethyl)terephthalate in the presence of the immobilized TfCa |
| 3.1.1.101 | poly(ethylene terephthalate) hydrolase |
degradation |
a dual enzyme system consisting of the polyester hydrolase and the immobilized carboxylesterase TfCa from Thermobifida fusca KW3 can be employed for the hydrolysis of PET films at 60°C, resulting in an increased amount of soluble products with a lower proportion of mono-(2-hydroxyethyl)terephthalate in the presence of the immobilized TfCa. The dual enzyme system with LC-cutinase produces a 2.4fold higher amount of degradation products compared to Thermobifida fusca enzyme Cut2 after a reaction time of 24 h |
| 3.2.1.8 | endo-1,4-beta-xylanase |
degradation |
a fungal consortium of Aspergillus nidulans, Mycothermus thermophilus, and Humicola sp. composts a mixture (1:1) of silica rich paddy straw and lignin rich soybean trash during summer period in North India, results in a product with C:N ratio 9.5:1, available phosphorus 0.042% and fungal biomass 6.512 mg of N-acetyl glucosamine/100 mg of compost. A C:N ratio of 10.2:1 and highest humus content of 3.3% is achieved with 1:1 mixture of paddy straw and soybean trash. The consortium shows high cellobiase, carboxymethyl cellulase, xylanase, and FPase activities |
| 3.2.1.4 | cellulase |
degradation |
a fungal consortium of Aspergillus nidulans, Mycothermus thermophilus, and Humicola sp. composts a mixture (1:1) of silica rich paddy straw and lignin rich soybean trash during summer period in North India, results in a product with C:N ratio 9.5:1, available phosphorus 0.042% and fungal biomass 6.512 mg of N-acetyl glucosamine/100 mg of compost. A C:N ratio of 10.2:1 and highest humus content of 3.3% is achieved with 1:1 mixture of paddy straw and soybean trash. The consortium shows showed high cellobiase, carboxymethyl cellulase, xylanase, and FPase activities |
| 3.2.1.176 | cellulose 1,4-beta-cellobiosidase (reducing end) |
degradation |
a fungal consortium of Aspergillus nidulans, Mycothermus thermophilus, and Humicola sp. composts a mixture (1:1) of silica rich paddy straw and lignin rich soybean trash during summer period in North India, results in a product with C:N ratio 9.5:1, available phosphorus 0.042% and fungal biomass 6.512 mg of N-acetyl glucosamine/100 mg of compost. A C:N ratio of 10.2:1 and highest humus content of 3.3% is achieved with 1:1 mixture of paddy straw and soybean trash. The consortium shows showed high cellobiase, carboxymethyl cellulase, xylanase, and FPase activities |
| 3.4.19.12 | ubiquitinyl hydrolase 1 |
degradation |
a natural dodecapeptide amide from UCH-L3 with the sequence DPDELRFNAIAL is capable of binding to monoubiquitin and may enable the design of peptides with different affinities towards K48- and K63-linked polyubiquitin |
| 1.1.1.284 | S-(hydroxymethyl)glutathione dehydrogenase |
degradation |
a Saccharomyces cerevisiae mutant lacking the gene for S-hydroxymethylglutathione dehydrogenase and expressing FldA is able to degrade 4 mM formaldehyde within 30 h |
| 3.1.8.1 | aryldialkylphosphatase |
degradation |
a series of substituted phenoxyalkyl pyridinium oximes enhance the degradation of surrogates of sarin (i.e. nitrophenyl isopropyl methylphosphonate, NIMP) and VX (i.e. nitrophenyl ethyl methylphosphonate, NEMP). Neither NIMP nor NEMP is hydrolyzed effectively by paraoxonase PON1 if one of these oximes is absent. In the presence of eight novel oximes, PON1-mediated degradation of both surrogates occurs |
| 2.3.1.184 | acyl-homoserine-lactone synthase |
degradation |
a significant positive correlation is observed between isoform LasI expression and polycyclic aromatic hydrocarbon degradation. Expression of isoform LasI increases with increase in biofilm growth, while the expression of isoform RhlI decreases during log phase of biofilm growth. Degradation of phenanthrene and pyrene by Pseudomonas aeruginosa N6P6 is affected by biofilm growth and LasI expression. The respective phenanthrene degradation for 15, 24, 48, and 72 h old biofilm after 7 days is 21.5, 54.2, 85.6, and 85.7%. The corresponding pyrene degradation is 15, 18.28, 47.56, and 46.48%, respectively, after 7 days |
| 3.2.1.4 | cellulase |
degradation |
a statistical optimization approach involving Plackett-Burman design and response surface methodology on submerged fermentation using cane molasses medium results in the production of 72410, 36420, 32420 and 5180 U/l of xylanase, endo-beta-1,4-glucanase, exo-beta-1,4-glucanase, and beta-glucosidase, respectively, i.e. more than fourfold improvements in production of xylanolytic and cellulolytic enzymes. Addition of microparticles engineers fungal morphology and enhances enzymes production. Maximum sugar yield of 578.12 and 421.79 mg/g substrate for waste tea cup and rice straw, respectively, are achieved after 24 h |
| 3.4.19.1 | acylaminoacyl-peptidase |
degradation |
AAP displays both exo- and endopeptidase activities |
| 3.2.1.8 | endo-1,4-beta-xylanase |
degradation |
Abf51A shows greater synergistic effect in combination with xylanase (2.92fold) on wheat arabinoxylan degradation than other reported enzymes, the amounts of arabinose, xylose, and xylobiose are all increased in comparison to that by the enzymes acting individually |
| 3.2.1.55 | non-reducing end alpha-L-arabinofuranosidase |
degradation |
Abf51A shows greater synergistic effect in combination with xylanase (2.92fold) on wheat arabinoxylan degradation than other reported enzymes, the amounts of arabinose, xylose, and xylobiose are all increased in comparison to that by the enzymes acting individually |
| 3.1.1.75 | poly(3-hydroxybutyrate) depolymerase |
degradation |
About 8.58 g/l (R)-3-hydroxybutanoate is obtained after 8 h of incubation using extracellular PHB. The optimal conditions are 50°C and pH 8. Presence of CaCO3 increases (R)-3-hydroxybutanoate yields to 23.97 g/l by stabilizing the pH of the reaction system |
| 3.4.24.82 | ADAMTS-4 endopeptidase |
degradation |
abrasion of cartilage aggrecan in rheumatoid arthritis and osteoarthritis |
| 1.3.3.6 | acyl-CoA oxidase |
degradation |
ACO activity in Beauveria bassiana depends on the carbon source used for growth and the chain length of the substrate utilized for the oxidation reaction |
| 2.1.1.41 | sterol 24-C-methyltransferase |
degradation |
active site of the yeast SMT has the necessary amino acids to generate products common to SMT catalysis of plants and protozoa, minor perturbations in the active site topography brought about by mutagenesis are sufficient to recognize new substrates |
| 3.1.8.1 | aryldialkylphosphatase |
degradation |
activity and stability of organophosphorus hydrolase are enhanced by interactions between the hydrophobic poly(propylene oxide) block of amphiphilic Pluronics and the enzyme. The strategy provides an efficient route to new formulations for decontaminating organophosphate neurotoxins |
| 3.1.1.73 | feruloyl esterase |
degradation |
addition of a crude enzyme supernatant from high xylanase producing actinomycete strain Kitasatospora sp. ID06-480 and ethyl ferulate producing actinomycete strain Nonomuraea sp. ID06-094 to sugarcane bagasse hydrolysis with low-level loading of commercial enzyme Cellic® CTec2 enhances both the released amount of glucose and reducing sugars. High conversion yield of glucose from cellulose at 60.5% can be achieved after 72 h of saccharification |
| 3.2.1.21 | beta-glucosidase |
degradation |
addition of beta-glucosidase to the rice straw hydrolysis reaction containing a commercial cellulase results in increase of reducing sugars being released |
| 3.2.1.4 | cellulase |
degradation |
addition of Cel9K to a commercial enzyme set (Celluclast 1.5L + Novozym 188) increases the saccharification of the pretreated reed and rice straw powders by 30.4% and 15.9%, respectively |
| 3.2.1.4 | cellulase |
degradation |
addition of Eg5A to cellobiase (i.e. cellobiohydrolase and beta-glucosidase) results in a 53% increasing saccharification of NaOH-pretreated barley straw, and the glucose release is 47% higher than with cellobiase treatment alone |
| 1.14.13.231 | tetracycline 11a-monooxygenase |
degradation |
addition of Escherichia coli overexpressing TetX to soil bacterial enrichment cultures along with varying levels of tetracycline affects community-wide tetracycline resistance levels. Soil microbial communities develop lower levels of tetracycline resistance upon exposure to 25 microg/ml of tetracycline when an Escherichia coli expressing TetX is present (6% of cultivable bacteria are resistant to 40 microg/ml tetracycline). In the absence of TetX activity, a similar tetracycline exposure selects for greater levels of resistant bacteria in the soil microbial community (90% of cultivable bacteria are resistant to 40 microg/ml tetracycline) |
| 3.2.1.4 | cellulase |
degradation |
addition of isoform Eg5A to cellobiase (cellobiohydrolase and beta-glucosidase) results in a 53% increasing saccharification of NaOH-pretreated barley straw, whereas the glucose release is 47% higher than that cellobiase treatment alone |
| 3.2.1.4 | cellulase |
degradation |
addition of recombinant Eg5A to cellobiase (cellobiohydrolase and beta-glucosidase) results in a 53% increase in saccharification of NaOH-pretreated barley straw, whereas the glucose release is 47% higher than with cellobiase treatment alone |
| 3.4.13.9 | Xaa-Pro dipeptidase |
degradation |
advantages of using Alteromonas recombinant prolidase in biodecontamination foams due to its high activity against G-type nerve agents, such as soman and sarin |
| 3.5.1.9 | arylformamidase |
degradation |
aerobic degradation of L-tryptophan |
| 3.2.1.4 | cellulase |
degradation |
after hydrolysis and fermentation of wheat straw a significant amount of active enzymes can be recovered by recycling the liquid phase. In the early stage of the process, enzyme adsorbs to the substrate, then gradually returning to the solution as the saccharification proceeds. The hydrolysis yield and enzyme recycling efficiency in consecutive recycling rounds can be increased by using high enzyme loadings and moderate temperatures. The amount of enzymes in the liquid phase increases with its thermostability and hydrolytic efficiency |
| 3.2.1.8 | endo-1,4-beta-xylanase |
degradation |
agroresidues subjected to alkali and microwave irradiation for 6 min in order to expose the polysaccharide component to enzymatic hydrolysis lead to increased relase of sugars. The maximum sugar content is detected in the hydrolysate of microwave-irradiated wheat bran (6.20 mg/g substrate) followed by wheat straw (4.9 mg/g substrate) |
| 1.2.3.1 | aldehyde oxidase |
degradation |
aldehyde oxidase plays a critical role in nitrite reduction, and this process is regulated by pH, oxygen tension, nitrite, and reducing substrate concentrations |
| 1.2.1.36 | retinal dehydrogenase |
degradation |
ALDH1A1 appears to be the major if not the only enzyme responsible for the oxidation of 3-deoxyglucosone to 2-keto-3-deoxygluconate |
| 4.2.2.3 | mannuronate-specific alginate lyase |
degradation |
alginate lyase is probably not suitable for hydrolysis of microcapsules in the presence of cells, in order to achieve high cell density and high productivity. However, the high activity may be useful for releasing cells from alginate beads or AG/PLL microcapsules |
| 3.2.1.139 | alpha-glucuronidase |
degradation |
an enzymatic cocktail consisting of Agu115 with xylanase (Xyn10C), an alpha-L-arabinofuranosidase (AbfA), and a beta-xylosidase (XynB) achieves almost complete conversion of glucuronoarabinoxylan to arabinofuranose, xylopyranose, and methyl glucuronate monosaccharides. Addition of isoform Agu115 to the enzymatic cocktail contributes specifically to 25% of the conversion |
| 3.2.1.8 | endo-1,4-beta-xylanase |
degradation |
application in enzymatic hydrolysis for sugars production from lignocellulosic biomass. On empty fruit bunch as a feedstock, the total sugars conversion is 3.8%, and the conversion after alkaline pretreatment is approximately 16fold improved (61.1%) |
| 3.2.1.8 | endo-1,4-beta-xylanase |
degradation |
application of enzyme for biobleaching of Eucalyptus kraft pulp, the xylanase increases the brightness of the pulp by 14.5% and reduces the kappa number by 24.5% |
| 3.2.1.21 | beta-glucosidase |
degradation |
application of enzyme in fed-batch hydrolysis of cellulose and high-temperature simultaneous saccharification and fermentation. beta-Glucosidase is suitable for lignocellulose conversion into ethanol |
| 1.11.1.6 | catalase |
degradation |
application of KatA for elimination of H2O2 after cotton fabrics bleaching leads to less consumption of water, steam and electric power by 25%, 12% and 16.7% respectively without productivity and quality loss of cotton fabrics |
| 3.2.1.91 | cellulose 1,4-beta-cellobiosidase (non-reducing end) |
degradation |
application of recombinant CBH II in hydrolysis of corn stover and rice straw pretreated with sodium hydroxide by improving the exo-exosynergism between CBH II and CBH I in Hypocrea jecorina. The yields 94.7% and 83.3% are achieved in the enzymatic hydrolysis of corn stover and rice straw pretreated by sodium hydroxide, respectively |
| 1.8.1.21 | dissimilatory dimethyldisulfide reductase |
degradation |
application of strain in a two-stage biotrickling filter for simultaneous treatment of hydrogen sulfide, methanethiol, dimethyl sulfide and dimethyl disulfide. The first biofilter is inoculated with Acidithiobacillus thiooxidans and the second one with Thiobacillus thioparus. For separate feeds of reduced sulfur compounds, the elimination capacity order is dimethyl disulfide > dimethyl sulfide > methanethiol |
| 3.2.1.55 | non-reducing end alpha-L-arabinofuranosidase |
degradation |
application potential for industrial purposes, biomass degradation and refining, extremely low end-product inhibition by arabinose further increases its applicability |
| 3.1.8.2 | diisopropyl-fluorophosphatase |
degradation |
approach to the use of enzyme encapsulated within liposomes, to protect against and treat chemical poisoning |
| 2.7.2.2 | carbamate kinase |
degradation |
arginine is metabolized by the arginine deiminase pathway |
| 3.4.21.61 | Kexin |
degradation |
ASP preferentially cleaves the peptide bond following two basic residues, one of which is Lys, but not the bond following a single basic residue. Tertiary structure around the catalytic domain of ASP resembles, but is not identical to that of furin |
| 3.1.1.101 | poly(ethylene terephthalate) hydrolase |
degradation |
at 50°C, a maximum hydrolysis rate for poly(ethylene terephthalate) nanoparticles of 0.0033 per min is determined with 80 microg/ml of Tcur_1278. With 50 microg/ml of Tcur_1278, the hydrolysis rate increases 1.8fold at 55°C and 2.6fold at 60°C |
| 3.1.1.101 | poly(ethylene terephthalate) hydrolase |
degradation |
at 50°C, a maximum hydrolysis rate of poly(ethylene terephthalate) nanoparticles of 0.0059 per min is determined with 20 microg/ml of Tcur_0390 |
| 1.4.1.2 | glutamate dehydrogenase |
degradation |
at high salinity glutamate seems to be preferentially produced through the process catalyzed by NADH-GDH, whereas GS-catalysis might be the main glutamate synthesis pathway under low salinity |
| 2.1.1.137 | arsenite methyltransferase |
degradation |
Bacillus subtilis 168 expressing ArsM converts most of the inorganic As in the medium into dimethylarsenate and trimethylarsine oxide within 48 h and volatizes substantial amounts of dimethylarsine and trimethylarsine. The rate of As methylation and volatilization increases with temperature from 37 to 50°C. When inoculated into an As-contaminated organic manure composted at 50°C, the modified strain significantly enhances As volatilization |
| 1.97.1.1 | chlorate reductase |
degradation |
bacterial reduction of chlorate and perchlorate in water |
| 1.13.11.1 | catechol 1,2-dioxygenase |
degradation |
because of broad spectrum of dioxygenases types that Stenotrophomonas maltophilia KB2 can exhibit, this strain appears to be very powerful and useful tool in the biotreatment of wastewaters and in soil decontamination |
| 1.13.11.2 | catechol 2,3-dioxygenase |
degradation |
because of broad spectrum of dioxygenases types that Stenotrophomonas maltophilia KB2 can exhibit, this strain appears to be very powerful and useful tool in the biotreatment of wastewaters and in soil decontamination |
| 1.13.11.3 | protocatechuate 3,4-dioxygenase |
degradation |
because of broad spectrum of dioxygenases types that Stenotrophomonas maltophilia KB2 can exhibit, this strain appears to be very powerful and useful tool in the biotreatment of wastewaters and in soil decontamination |
| 3.2.1.21 | beta-glucosidase |
degradation |
beta-glucan can be completely degraded to glucose at high temperature with a combination of the hyperthermophile Pyrococcus furiosus endocellulase (EGPf) and beta-glucosidase (BGLPf). beta-Glucans are polysaccharides of D-glucose monomers formed by beta(1->3),(1->4) mixed-linkage bonds. They occur most commonly as cellulose in plants, in the bran of cereal grains, the cell wall of baker's yeast, and in certain fungi, mushrooms, and bacteria |
| 3.1.1.101 | poly(ethylene terephthalate) hydrolase |
degradation |
bioconversion of plastics |
| 3.1.1.101 | poly(ethylene terephthalate) hydrolase |
degradation |
biodegradability of PET is mainly influenced by the mobility of the polyester chains, which determine the affinity and accessibility of the ester bonds to the enzyme. The hydrolysis rates of enzymatic PET degradation are predominantly controlled by the efficient substrate adsorption rather than by the hydrolysis of the ester bonds. Nanoparticles prepared from PET samples of different crystallinity show a high proportion of amorphous domains and thus in the corresponding biodegradability |
| 3.2.1.4 | cellulase |
degradation |
bioethanol production by Aspergillus fumigatus JCF at optimised growth conditions and Saccharomyces cerevisiae for simultaneous saccharification and fermentation. Using cotton seed as the substrate, maximum bioethanol concentration of 6.7 g/l can be achieved |
| 1.11.1.13 | manganese peroxidase |
degradation |
biomimmetic decrosslinking with enzyme or metal complex-catalyzed reactions will enable the development of new devulcanizing strategies for the safe disposal and recycling of waste vulcanized rubber products |
| 3.1.1.74 | cutinase |
degradation |
biotechnological applications of cutinases for synthetic polyester degradation |
| 3.5.4.42 | N-isopropylammelide isopropylaminohydrolase |
degradation |
biotic interaction between earthworms and the bacterial community involved in degradation of the herbicide atrazine in a maize-cropped soil, earthworms significantly affect the structure of the soil bacterial communities in the biostructures, they reduce the size of the population of Pseudomonas sp. ADP, thereby contributing to the diminution of the atrazine-degrading genetic potential in soil microsites |
| 1.10.3.2 | laccase |
degradation |
bisphenol A degradation |
| 3.5.4.42 | N-isopropylammelide isopropylaminohydrolase |
degradation |
broad level bacterial community interactions that are involved in atrazine degradation in nature |
| 3.5.4.42 | N-isopropylammelide isopropylaminohydrolase |
degradation |
broad level bacterial community interactions that are involved in atrazine degradation in nature, Nocardia sp. plays a crucial role for stable maintenance of the degrader community, dechlorination of atrazine is carried out exclusively by Nocardia sp. which apart from the atzC gene contains the trzN gene |
| 3.1.1.73 | feruloyl esterase |
degradation |
capable of decolourising effluent from the paper industry, potential application in obtaining ferulic acid from agriculture waste materials produced by milling, brewing and sugar industries |
| 1.1.99.18 | cellobiose dehydrogenase (acceptor) |
degradation |
CDH is able to produce a sufficient amount of H2O2 to decolorize anthocyanins within 2 h |
| 3.2.1.4 | cellulase |
degradation |
cellulase complex containing cellulolytic enzymes,endoglucanase CelE, EC 3.2.1.4, and beta-glucosidase BglA, EC 3.2.1.21, to completely degrade cellulose to glucose. The cellulases are displayed on the cell surface of Corynebacterium glutamicum by using themechanosensitive channel to anchor enzymes in the cytoplasmic membrane. The displayed cellulases complexes have a synergic effect on the direct conversion of biomass to reducing sugars leading to 3.1- to 6.0fold increase compared to the conversion by the secreted cellulases complexes. The displayed cellulases complexes increase the residual activities of cCelEand cBglA at 70°C from 28.3% and 24.3% in the secreted form to 65.1% and 82.8%, respectively |
| 3.2.1.4 | cellulase |
degradation |
cellulase enzyme filtrate from Chaetomium thermophile saccharifies 5% kallar grass straw to 69% reducing sugars (quantitatively) at 50°C. Glucose concentration in the hydrolysates from different fungi is in the decreasing order of Chaetomium thermophile > Trichoderma reesei > Sporotrichum thermophile > Aspergillus fumigatus > Torula thermophila > Humicola grisea > Malbranchea pulchella. At 60°C, thermostable enzymes hydrolyse kallar grass straw at a maximum rate for the initial 20 h |
| 3.2.1.4 | cellulase |
degradation |
cellulase is an industrially important enzyme for biomass saccharification at high temperature. beta-Glucan can be completely degraded to glucose at high temperature with a combination of the hyperthermophile Pyrococcus furiosus endocellulase (EGPf) and beta-glucosidase (BGLPf). beta-Glucans are polysaccharides of D-glucose monomers formed by beta(1->3),(1->4) mixed-linkage bonds. They occur most commonly as cellulose in plants, in the bran of cereal grains, the cell wall of baker's yeast, and in certain fungi, mushrooms, and bacteria |
| 1.14.99.54 | lytic cellulose monooxygenase (C1-hydroxylating) |
degradation |
cellulose conversion by cellobiohydrolase Cel7A from Trichoderma longibrachiatum alone is enhanced from 46 to 54% by the addition of isoform AA9A. Conversion by a mixture of Cel7A, endoglucanase, and beta-glucosidase is increased from 79 to 87% using pretreated bacterial microcrystalline cellulose with AA9A for 72 h. Individual AA9A molecules exhibit intermittent random movement along, across, and penetrating into the ribbon-like microfibril structure of bacterial microcrystalline cellulose, concomitant with the release of a small amount of oxidized sugars and the splitting of large cellulose ribbons into fibrils with smaller diameters |
| 1.4.1.2 | glutamate dehydrogenase |
degradation |
clarification of the in vivo direction of the reaction catalyzed by GDH isoenzyme 1, the enzyme catabolizes L-glutamate in roots, and does not assimilate NH4+ in source leaves |
| 3.4.21.7 | plasmin |
degradation |
cleavage at Arg336 is a central mechanism of plasmin-catalyzed factor VIII inactivation. Cleavages at Arg336 and Lys36 are selectively regulated by the A2 and A3-C1-C2 domains, respectively, interacting with plasmin |
| 3.2.1.8 | endo-1,4-beta-xylanase |
degradation |
co-immobilization of xylanase, beta-xylosidase and alpha-L-arabinofuranosidase from Penicillium janczewskii on a single support leads to a functional multi-enzymatic biocatalyst acting in the complete hydrolysis of different and complex substrates such as oat spelt and wheat arabinoxylans, with xylose yield higher than 40%. The xylanase and the alpha-L-arabinofuranosidase present high stability retaining 86.6 and 88.0% of activity after 10 reuse cycles |
| 3.2.1.37 | xylan 1,4-beta-xylosidase |
degradation |
co-immobilization of xylanase, beta-xylosidase and alpha-L-arabinofuranosidase from Penicillium janczewskii on a single support leads to a functional multi-enzymatic biocatalyst acting in the complete hydrolysis of different and complex substrates such as oat spelt and wheat arabinoxylans, with xylose yield higher than 40%. The xylanase and the alpha-L-arabinofuranosidase present high stability retaining 86.6 and 88.0% of activity after 10 reuse cycles |
| 3.2.1.55 | non-reducing end alpha-L-arabinofuranosidase |
degradation |
co-immobilization of xylanase, beta-xylosidase and alpha-L-arabinofuranosidase from Penicillium janczewskii on a single support leads to a functional multi-enzymatic biocatalyst acting in the complete hydrolysis of different and complex substrates such as oat spelt and wheat arabinoxylans, with xylose yield higher than 40%. The xylanase and the alpha-L-arabinofuranosidase present high stability retaining 86.6 and 88.0% of activity after 10 reuse cycles |
| 1.6.2.4 | NADPH-hemoprotein reductase |
degradation |
coexpression of CPR and P450 cytochromes CYP6AE14 or CYP9A12 of Helicoverpa armigera in Pichia pastoris. CYP6AE14 is not involved in gossipol degradation, but CYP9A12 takes part in gossipol metabolism |
| 1.14.13.243 | toluene 2-monooxygenase |
degradation |
coexpression of subunit TomA3 mutant V106A and an engineered epoxide hydrolase EchA from Agrobacterium radiobacter AD1, enhances the degradation of cis-dichloroethylene |
| 3.2.1.91 | cellulose 1,4-beta-cellobiosidase (non-reducing end) |
degradation |
combined use of isoforms CBH A and Cbh C on degradation of cotton. Conversion after 72 h is about 19 % by weight, with an almost fourfold increase in enzymatic hydrolysis yield by intermittent product removal of cellobiose with membrane filtration. A synergistic effect, achieving about 27 % substrate conversion, is obtained by addition of endo-1,4-beta-D-glucanase |
| 4.2.2.26 | oligo-alginate lyase |
degradation |
combining exotype alginate lyases OalC6 and OalC17 and the endotype alginate lyase AlySY08 enables the production of alginate monomers due to synergistic processes |
| 3.2.1.176 | cellulose 1,4-beta-cellobiosidase (reducing end) |
degradation |
commercial cellulases contain mannan hydrolysing enzymes. Addition of 10 mg/ml mannan reduces the glucose yield of avicel (at 20 g/l) from 40.1 to 24.3%. The inhibitory effect is at least partly attributed to the inhibition of Cel7A(CBHI), but not on beta-glucosidase |
| 1.13.11.2 | catechol 2,3-dioxygenase |
degradation |
comparison of binding sites and affinities using substrates chlorsulfon and metsulfuron-methyl. Homoprotocatechuate 2,3-dioxygenase from Brevibacterium fuscum and Arthrobacter globiformis are more effective in binding than catechol 2,3-dioxygenase from Pseudomonas putida. B. fuscum and A. globiformis have more potential than P. putida to remediate chlorsulfuron and metsulfuronmethyl |
| 1.13.11.15 | 3,4-dihydroxyphenylacetate 2,3-dioxygenase |
degradation |
comparison of binding sites and affinities using substrates chlorsulfon and metsulfuron-methyl. Homoprotocatechuate 2,3-dioxygenase from Brevibacterium fuscum and Arthrobacter globiformis are more effective in binding than catechol 2,3-dioxygenase from Pseudomonas putida. B. fuscum and A. globiformis have more potential than P. putida to remediate chlorsulfuron and metsulfuronmethyl |
| 3.2.1.4 | cellulase |
degradation |
comparison of endoglucanases able to rapidly reduce the viscosity of 15% (w/w, dry matter) hydrothermally pretreated wheat straw. Based on temperature profiling studies, Thermoascus aurantiacus EGII/Cel5A is the most promising enzyme for biomass liquefaction |
| 3.2.1.176 | cellulose 1,4-beta-cellobiosidase (reducing end) |
degradation |
comparison of the activity w ith Humicola jecorina Cel7A reveals a much higher hydrolytic rate for Humicola grisea Cel7A at both 65°C (4.8fold higher initial rate) and 38°C (3.3fold higher) |
| 1.8.1.4 | dihydrolipoyl dehydrogenase |
degradation |
conservation of the Cys-45 residue in human E3 is essential to the efficient catalytic function of the enzyme |
| 3.2.1.91 | cellulose 1,4-beta-cellobiosidase (non-reducing end) |
degradation |
construction of a consolidated bioprocessing-enabling yeast by constitutive expression of genes Cbh1 from Aspergillus aculeatus, Cbh1 and Cbh2 from Hypocrea jecorina. Additionally, Hypocrea jecorina Eg2, Aspergillus aculeatus Bgl1 are integrated into the Saccharomyces cerevisiae chromosome. The resultant strains expressing uni-, bi-, and trifunctional cellulases, respectively, exhibit corresponding cellulase activities and both the activities and glucose producing activity ascends. Evaluation in acid- and alkali-pretreated corncob containing media with 5 FPU exogenous cellulase/g biomass loading shows that compared with the control strains, the engineered strains efficiently ferment pretreated corncob to ethanol |
| 3.2.1.176 | cellulose 1,4-beta-cellobiosidase (reducing end) |
degradation |
construction of a consolidated bioprocessing-enabling yeast by constitutive expression of genes Cbh1 from Aspergillus aculeatus, Cbh1 and Cbh2 from Hypocrea jecorina. Additionally, Hypocrea jecorina Eg2, Aspergillus aculeatus Bgl1 are integrated into the Saccharomyces cerevisiae chromosome. The resultant strains expressing uni-, bi-, and trifunctional cellulases, respectively, exhibit corresponding cellulase activities and both the activities and glucose producing activity ascends. Evaluation in acid- and alkali-pretreated corncob containing media with 5 FPU exogenous cellulase/g biomass loading shows that compared with the control strains, the engineered strains efficiently ferment pretreated corncob to ethanol |
| 1.2.1.72 | erythrose-4-phosphate dehydrogenase |
degradation |
coupling of a transketolase reaction (using Leishmania mexicana transketolase) that converts D-fructose 6-phosphate to D-erythrose 4-phosphate, which can then be converted to 4-phosphate D-erythronate using E4PD, whereby D-ribose 5-phosphate and D-glyceraldehyde 3-phosphate can both be used as ketol acceptor substrates in the reaction |
| 3.2.1.11 | dextranase |
degradation |
crosslinking of dextranase on chitosan hydrogel microspheres. A shift in optimum pH and temperature from 7.0 to 7.5 and 50 to 60°C is observed after immobilization, respectively. Recycling efficiency, thermal stability, and activation energy distinctly improve after immobilization, whereas anchoring of substrate at the active site of the soluble dextranase exhibits an increase in Km with no change in Vmax after crosslinking |
| 3.2.1.4 | cellulase |
degradation |
crude cellulase efficiently hydrolyzes office waste paper, algal pulp (Gracillaria verulosa), and biologically treated wheat straw at 60°C with sugar release of about 830 mg/ml, 285 mg/g, and 260 mg/g of the substrate, respectively |
| 1.11.1.13 | manganese peroxidase |
degradation |
crude enzyme is able to degrade the antibiotics tetracycline and oxytetracycline. 72.5% of 50 mg/l of tetracycline and 84.3% of 50 mg/l oxytetracycline is degraded by 40 U/l of amnganese peroxidase, within 4 h. With the pH at 3.0-4.8, the temperature at 37-40°C, the Mn2+ concentration between 0.1 and 0.4 mM, the H2O2 concentration of 0.2 mM, and the enzyme-substrate ratio above 2.0 U/mg, the degradation rate reaches the highest |
| 3.2.1.4 | cellulase |
degradation |
crude thermostable cellulases and xylanase hydrolyze phosphoric acid-swollen wheat straw, avicel and untreated xylan up to 74, 71 and 90 %, respectively |
| 3.2.1.8 | endo-1,4-beta-xylanase |
degradation |
crude thermostable cellulases and xylanase hydrolyze phosphoric acid-swollen wheat straw, avicel and untreated xylan up to 74, 71 and 90 %, respectively |
| 1.10.3.2 | laccase |
degradation |
cyanobacterial laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. Due to phototrophic mode of nutrition, short generation time and easy mass cultivation, Spirulina platensis laccase appears as good candidate for laccase production. The high yield of laccase in short production period are profitable for its industrial application. Pure Spirulina platensis laccase alone can efficiently decolorized anthraquinonic dye Reactive Blue 4 without any mediators which makes it cost effective and suitable candidate for decolorization of synthetic dyes and help in waste water treatment |
| 3.4.24.78 | gpr endopeptidase |
degradation |
D127 and D193 are essential for activity and autoprocessing |
| 1.10.3.2 | laccase |
degradation |
decolorization of industrial dyes. Evans blue decolorization and detoxification |
| 1.8.1.4 | dihydrolipoyl dehydrogenase |
degradation |
decreased activity of DLDH induced by valproic acid metabolites may, at least in part, account for the impaired rate of oxygen consumption and ATP synthesis in mitochondria if 2-oxoglutarate or glutamate are used as respiratory substrates, thus limiting the flux of these substrates through the citric acid cycle |
| 3.2.1.8 | endo-1,4-beta-xylanase |
degradation |
degradation of alpha-amylase-treated wheat bran by xylanase solubilises about 20% of the fibre residue, i.e. 10% of the original bran. Amylase, protease and xylanase treatments alter the composition of the original bran, removing starch (100%), a portion of the non-starch glucan (39%), xylan (57%), arabinan (61%), ash (62%) and other components including protein (52%). Pre-extraction of enzymatically-hydrolysable starch and xylan reduces the release of furfural. Steam explosion of the lignocellulosic residue followed by cellulase treatment and conversion to ethanol at a high substrate concentration (19%) gives an ethanol titre ofabout 25 g/l or a yield of 93% of the theoretical maximum |
| 3.4.24.82 | ADAMTS-4 endopeptidase |
degradation |
degradation of cartilage in late-stage Lyme arthritis |
