EC Number   |
Recommended Name  |
Application |
|---|
    1.1.1.188 | prostaglandin-F synthase |
molecular biology |
genome editing of human cell lines can be used to complement mouse KO models to validate the function of genes in differentiated tissues and cells |
| 1.1.1.219 | dihydroflavonol 4-reductase |
molecular biology |
the gene encoding dihydroflavonol 4-reductase is a candidate for the anthocyaninless locus of rapid cycling Brassica rapa (fast plants type) |
| 1.1.1.22 | UDP-glucose 6-dehydrogenase |
molecular biology |
the high activity combined with the simple purification procedure used make GbUGD a valuable alternative biocatalyst for the synthesis of UDP-glucuronic acid or the development of NAD+ regeneration systems |
| 1.1.1.28 | D-lactate dehydrogenase |
molecular biology |
D-lactate dehydrogenase is useful as a marker gene allowing positive selection of transgenic plants |
| 1.1.1.34 | hydroxymethylglutaryl-CoA reductase (NADPH) |
molecular biology |
Lactococcus lactis is a potential heterologous host for the production of sesquiterpenes from a herbaceous Malaysian plant, Persicaria minor. A sesquiterpene synthase gene encoding beta-sesquiphellandrene synthase from Persicaria minor is successfully cloned and expressed in Lactococcus lactis. Overexpression of the Lactococcus lactis endogenous 3-hydroxy-3-methylglutaryl Co-A reductase, an established rate-limiting enzyme in the eukaryotic mevalonate pathway, increases the production level of beta-sesquiphellandrene by 1.25-1.60 fold |
| 1.1.1.6 | glycerol dehydrogenase |
molecular biology |
enzymatic redox cofactor regeneration in organic media: functionalization and application of recombinant glycerol dehydrogenase and soluble transhydrogenase in reverse micelles, overview |
| 1.1.1.9 | D-xylulose reductase |
molecular biology |
a high thermostability of PsXDH is obtained by subsequent site-directed mutagenesis of the structural zinc-binding loop. The best mutant in this study (C4/F98R/E101F) shows a 10.8 °C higher thermal transition temperature and 20.8 °C higher half denaturation temperature (T1/2) compared with wild-type |
| 1.1.1.95 | phosphoglycerate dehydrogenase |
molecular biology |
results demonstrate that PGDH enhances the levels of betaine by providing the precursor serine for both choline oxidation and glycine methylation pathways |
| 1.1.1.95 | phosphoglycerate dehydrogenase |
molecular biology |
results demonstrate that the promoter activity of the human PHGDH gene is positively regulated by the action of transcription factors Sp1 and NF-Y |
| 1.1.3.13 | alcohol oxidase |
molecular biology |
Pichia pastoris is an efficient host for the expression and secretion of heterologous proteins possessing a strong and tightly regulated promoter from the alcohol oxidase I, AOX1, gene. The transformed cells need to be activated by methanol and grow on methanol as carbon source. With the inducible AOX1 promoter an increase of the copy number above two resulted in a decrease of expression. Combined use of GAP and AOX1 promoters in Pichia pastoris, overview |
| 1.1.3.15 | (S)-2-hydroxy-acid oxidase |
molecular biology |
the interaction of capsid protein P8 with the GOX of host cells leads to translocation of capsid protein P8 into peroxisomes. The interaction between capsid protein P8 and GOX plays important roles in Rice dwarf phytoreovirus targeting into the replication site of host cells |
| 1.1.3.8 | L-gulonolactone oxidase |
molecular biology |
short-term vitamin A deficiency in broiler chicks reduces GULO activity without concomittant changes in tissue ascorbic acid |
| 1.1.3.9 | galactose oxidase |
molecular biology |
glycoprotein labeling using engineered variants of galactose oxidase, overview |
| 1.1.98.7 | Ser-type anaerobic sulfatase-maturating enzyme |
molecular biology |
enzymatic modification of a DARPin model protein carrying the aldehyde tag sequence SAPAR enables the production of a fluorescent conjugate. Thus proving its potential for bioconjugation chemistry. This tag system could be useful in orthogonal combination with the standard cysteine-type tag system targeted by formylglycine-generating enzyme, which is regularly utilized for protein labeling |
| 1.1.99.29 | pyranose dehydrogenase (acceptor) |
molecular biology |
gene expression analysis using PCR, pdh1 expression is upregulated upon exhaustion of the carbon source and appears to be additionally regulated under conditions of oxygen limitation, PDH production is highest on cellobiose and very low on fructose |
| 1.1.99.29 | pyranose dehydrogenase (acceptor) |
molecular biology |
gene expression analysis using PCR, pdh2 is constitutively expressed, PDH production is highest on cellobiose and very low on fructose |
| 1.1.99.29 | pyranose dehydrogenase (acceptor) |
molecular biology |
gene expression analysis using PCR, pdh3 is constitutively expressed, PDH production is highest on cellobiose and very low on fructose |
| 1.13.11.49 | chlorite O2-lyase |
molecular biology |
use of the heme enzyme for the rapid, in situ generation of O2 at concentrations far exceeding 2 mM in coupled enzyme reaction systems to study O consumption or O2 involving reaction steps. Catalytic concentrations of chlorite O2-lyase can be used to initiate the reaction of an O2-utilizing (metallo)enzyme by rapid mixing with the highly soluble, non-volatile ClO2, rather than with the sparingly soluble, gaseous O2, e.g. activation of the beta2 subunit of class Ic ribonucleotide reductase from Chlamydia trachomatis by mixing its MnII/FeII complex with ClO2- in the presence of chlorite O2-lyase, overview |
| 1.13.12.13 | Oplophorus-luciferin 2-monooxygenase |
molecular biology |
useful reporter protein in various assay systems including reporter assays and immunoassays |
| 1.13.12.13 | Oplophorus-luciferin 2-monooxygenase |
molecular biology |
a multicolor BRET assay for the quantification of global DNA methylation is developed using CXXC-fused Oplophorus luciferase (CXXC-Oluc), a methyl-CpG-binding domain-fused firefly luciferase (MBD-Fluc), BOBO-1, and BOBO-3. CXXC-Oluc recognized unmethylated CpG sites on genomic DNA to excite BOBO-1 DNA intercalating dye, whereas MBD-Fluc recognized methylated CpG sites on genomic DNA to excite BOBO-3 DNA intercalating dye. The emission intensities of BOBO-1 and BOBO-3 are simultaneously detected and depended on the unmethylated and methylated CpG contents of the genomic DNA. There is a significant negative correlation between the emission intensities of BOBO-1 and BOBO-3. Therefore, the global DNA methylation level can be quantified with this multicolor BRET assay using a single tube |
| 1.13.12.13 | Oplophorus-luciferin 2-monooxygenase |
molecular biology |
bioluminescence imaging is a powerful, broadly utilized method for non-invasive imaging studies in cell-based assays and small animal models of normal physiology and multiple diseases. In combination with molecular engineering of cells and entire organisms using luciferase enzymes, bioluminescence imaging has enabled novel applications including studies of protein-protein interactions, ligand-receptor interactions, cell trafficking, and drug targeting in mouse models. The use of a luciferase enzyme derived from Oplophorus gracilirostris, NanoLuc, is described in cell-based assays bioluminescence imaging of tumor-bearing mice. NanoLuc is combined with another luciferase enzyme, firefly luciferase, to image multiple signal transduction events in one imaging session |
| 1.13.12.5 | Renilla-type luciferase |
molecular biology |
development of a reverse genetic model to characterize the pathway of replication and pathogenesis of the SARS coronavirus. Renilla luciferase is used as a reporter gene and inserted into the backbone of the infectious clone of SARS coronavirus to replace ORF 7a/b (SARS wt-Luc), which is believed to have apoptotic effects on host cells. Recombinant viruses with luciferase constructs are isolated and shown to stably maintain the Renilla luciferase gene and to express subgenomic mRNA encoding luciferase. SARS wt-Luc is a viable virus that allows studies of the effect of subgenomic manipulation on virus efficiacy, both in replication and subgenomic production. This approach offers an alternative to plaque assay analysis in testing the efficiency of anti-SARS agents |
| 1.13.12.5 | Renilla-type luciferase |
molecular biology |
dual luciferase enzyme assay system for reporter gene analysis combining both the firefly luciferase enzyme and the Renilla luciferase enzyme in a nonproprietary buffer |
| 1.13.12.5 | Renilla-type luciferase |
molecular biology |
modification of the photoprotein aequorin by attaching selected fluorophores at a unique site on the protein. This will allow for in vitro transfer of bioluminescent energy from aequorin to the fluorophore thus creating an artificial jellyfish. The fluorophores are selected such that the excitation spectrum of the fluorophore overlaps with the emission spectrum of aequorin. By modifying aequorin with different fluorophores, bioluminescent labels with different emission maxima are produced, which will allow for the simultaneous detection of multiple analytes |
| 1.13.12.5 | Renilla-type luciferase |
molecular biology |
Renilla luciferase, fused to biospecific sequences such as engineered antibodies, can be administered systemically to provide a novel, sensitive method for optical imaging based on expression of cell surface receptors in living organism |
| 1.13.12.5 | Renilla-type luciferase |
molecular biology |
the development of variants of Renilla luciferase, which exhibit significantly improved properties compared with the native enzyme, will allow enhanced sensitivity in existing luciferase-based assays as well as enable the development of novel probes labeled with the luciferase protein |
| 1.13.12.5 | Renilla-type luciferase |
molecular biology |
when aequorin is microinjected into cleavage-stage zebrafish embryos, it is largely used up by about 24 hours. Thus, it is not possible to image Ca2+ signals from later stages of zebrafish development using this approach. Transient expression of apoaequorin (i.e., the protein component of aequorin) using aeq-mRNA in zebrafish embryos and then reconstitution of intact aequorin in vivo by loading the coelenterazine cofactor into the embryos separately provides a valuable tool for monitoring Ca2+ signaling during the 2448 h post fertilization period of zebrafish development. Thus, it effectively extends the aequorin-based Ca2+-imaging window by an additional 24 hours |
| 1.13.12.5 | Renilla-type luciferase |
molecular biology |
sensitive reproter for studies of gene expression, promoter activity, protein-protein interactions, signal transduction, tumor cell growth, response to therapy |
| 1.13.12.5 | Renilla-type luciferase |
molecular biology |
an advanced Fc-binding probe, FcUni-RLuc, is produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence HWRGWV is fused to Renilla luciferase, and the purified probe is employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells |
| 1.13.12.5 | Renilla-type luciferase |
molecular biology |
Renilla luciferase (Rluc) from Renilla reniformis is an appropriate protein reporter for the detection of specific molecular targets due to its bioluminescent feature, although its relatively low stability limits the application |
| 1.13.12.6 | Cypridina-luciferin 2-monooxygenase |
molecular biology |
construction of a cold-induced expression vector (pCold-ZZ-VL vector) in Escherichia coli cells that results in soluble bioluminescent fusion enzyme with binding ability to monoclonal antibodies, however, the Cypridina luciferase fusion enzyme is soluble but not bioluminescent (in contrast to other luciferases fused to the ZZ-domain of Staphylococcuss aureus protein A) |
| 1.13.12.6 | Cypridina-luciferin 2-monooxygenase |
molecular biology |
Renilla and Cypridina luciferases should be more appropriate tools for applications requiring the detection of small amounts of substrate as in small molecule detection assays because RLuc and CLuc respond to their luciferin concentration in a linear non-cooperative manner |
| 1.13.12.6 | Cypridina-luciferin 2-monooxygenase |
molecular biology |
Cypridina noctiluca luciferase is utilized for biochemical and molecular biological applications, including bioluminescent enzyme immunoassays, far-red luminescence imaging, and high-throughput reporter assays |
| 1.13.12.6 | Cypridina-luciferin 2-monooxygenase |
molecular biology |
luciferases can be used as light-emissive reporters of mechanoenzymatic reaction. The light emitted by a bioluminescent reaction can be used to directly monitor the progress of a mechanoenzymatic reaction without sampling |
| 1.13.12.6 | Cypridina-luciferin 2-monooxygenase |
molecular biology |
the thermostable enzyme can be used for various research applications, including in vivo imaging and high throughput reporter assays |
| 1.13.12.7 | firefly luciferase |
molecular biology |
Cypridina noctiluca luciferase is utilized for biochemical and molecular biological applications, including bioluminescent enzyme immunoassays, far-red luminescence imaging, and high-throughput reporter assays |
| 1.13.12.7 | firefly luciferase |
molecular biology |
firefly luciferase is widely used in molecular biology and bioanalytical systems as a reporter molecule due to the high quantum yield of the bioluminescence, availability of stable mutant forms of the enzyme with prescribed spectral characteristics and abundance of bacterial expression systems suitable for production of recombinant proteins in limitless quantities. Fusion proteins of luciferase are described with biotin-binding domain and treptavidin, with proteins A and G, antibodies, with DNA- and RNA-binding proteins, as well as fusion proteins designed for BRET systems. The firefly luciferase-based fusion proteins are represented as an effective tool for the development of different bioanalytical systems such as (1) systems in which luciferase is attached to the surface of the target and the bioluminescence signal is detected from the specific complexes formed, (2) BRET-based systems, in which the specific interaction induces changes in the bioluminescence spectrum, and (3) systems that use modified or split luciferases, in which the luciferase activity changes under the action of the analyte. All these systems have wide application in biochemical analysis of physiologically important compounds, for the detection of pathogenic bacteria and viruses, for evaluation of protein-protein interactions, assaying of metabolites involved in cell communication and cell signaling |
| 1.13.12.7 | firefly luciferase |
molecular biology |
Ppy luciferase can been used extensively as a reporter gene in living cells and organisms. Some biological applications are limited by the low stability of the luciferase and limited intracellular luciferin concentration. The mutant enzyme T214A/A215L/I232A/F295L/E345K/I423L/D436G/L530R exhibits both improved thermostability and brighter luminescence at low luciferin concentrations, it may be useful for reporter gene applications |
| 1.13.12.7 | firefly luciferase |
molecular biology |
the enzyme is a powerful tool for molecular and cellular biology, and popular in high-throughput screening and drug discovery |
| 1.14.11.1 | gamma-butyrobetaine dioxygenase |
molecular biology |
a fluorescence assay based on the detection of fluoride released from reactions of fluorinated substrates with BBOX by the use of tert-butyldimethylsilyl-protected fluorescein is developed. (3S)-3-fluoro-4-(trimethylammonio)butanoate is a good substrate for BBOX, releasing fluoride when subjected to the enzyme |
| 1.14.13.9 | kynurenine 3-monooxygenase |
molecular biology |
development of a transformant selection system for Tribolium castaneum on the basis of mutant rescue |
| 1.14.13.9 | kynurenine 3-monooxygenase |
molecular biology |
wild-type KMO gene can be used as a marker gene for visually screening transgenic silkworms |
| 1.14.13.9 | kynurenine 3-monooxygenase |
molecular biology |
the wild-type enzyme gene can be used as a marker gene for visually screening transgenic silkworms |
| 1.14.14.3 | bacterial luciferase |
molecular biology |
enzyme can be used to monitor changes in gene expression as a reporter system in slow-growing mycobacteria, i.e. Mycobacterium tuberculosis strain H37Ra, determination of recombinant enzyme decay rate |
| 1.14.14.3 | bacterial luciferase |
molecular biology |
the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview |
| 1.14.14.86 | ent-kaurene monooxygenase |
molecular biology |
the fungal cytochrome P450 monooxygenase isolated from from Phaeoshaeria sp., strain L487 in the Pichia pastoris expression system might become a tool for functional assays of a variety of rice cytochrome P450 monooxygenases involved in the biosynthesis of secondary metabolites |
| 1.14.16.1 | phenylalanine 4-monooxygenase |
molecular biology |
an automated fluorescence-based continuous real-time PAH activity assay that is faster and more efficient but as precise and accurate as standard methods is developed |
| 1.14.16.1 | phenylalanine 4-monooxygenase |
molecular biology |
bicistronic expression system is developed which allows the isolation of hybrid forms that exhibit negative interallelic complementation, and may represent a model system for studying the molecular pathogenic mechanisms of PAH gene mutations in compound heterozygous phenylketonuric patients, providing the rationale to understand the observed inconsistencies both in genotype/phenotype correlations and in the response to (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin supplementation |
| 1.17.1.4 | xanthine dehydrogenase |
molecular biology |
isozyme XDH1 has the potential to be used as a metabolic target for controlling populations of Aedes aegypti mosquitoes, which are vectors of public health threats |
| 1.17.4.1 | ribonucleoside-diphosphate reductase |
molecular biology |
titration of ribonucleotide reductase expression can serve as a standard model system for studying the coordination between chromosome replication, cell division, and cell size |
| 1.2.1.36 | retinal dehydrogenase |
molecular biology |
ALDH1A1 is rapidly gaining importance as a stem cell marker |
| 1.2.1.8 | betaine-aldehyde dehydrogenase |
molecular biology |
BADH application as a marker for chloroplast engineering without using antibiotic can avoid transferring antibiotic genes from the plant and thus assists to allay public concern regarding genetic modifications |
| 1.3.1.77 | anthocyanidin reductase [(2R,3R)-flavan-3-ol-forming] |
molecular biology |
a method for the analysis of ANR activity using the detection of coenzyme is established |
| 1.3.5.1 | succinate dehydrogenase |
molecular biology |
considering the conservation of amino acids crucial for the SQR activity and the high levels of ROS production from the mitochondrial complex II of the Ascaris suum adult worm together with the absence of complexes III and IV activities in its respiratory chain, it is a good model to examine the reactive oxygen species production from the mitochondrial complex II |
| 1.3.5.1 | succinate dehydrogenase |
molecular biology |
the iron-sulfur subunit (SdhB) of mitochondrial succinate dehydrogenase is encoded by a split and rearranged nuclear gene in Euglena gracilis. The two subgenic modules are transcribed independently and the resulting mRNAs appear to be independently translated, with the two protein products imported into mitochondria. The discovery of this unique molecular marker provides evidence for the monophyly of Euglenozoa that is independent of evolutionary models |
| 1.3.5.1 | succinate dehydrogenase |
molecular biology |
the relevance of modifications in Alternaria alternata AaSdhB sequence in conferring boscalid resistance is discussed |
| 1.3.5.1 | succinate dehydrogenase |
molecular biology |
the splitting of sdhB in Euglena and trypanosomatids is an example of a unique molecular character that specifically unites these two phylogenetic groups |
| 1.3.99.4 | 3-oxosteroid 1-dehydrogenase |
molecular biology |
development of a synthetic 3-ketosteroid DELTA1-dehydrogenase for the generation of a catabolic pathway enabling cholesterol degradation in human cells |
| 1.4.1.13 | glutamate synthase (NADPH) |
molecular biology |
GltA shows 73% identity to the corresponding protein of Oceanobacillus iheyensis |
| 1.4.1.13 | glutamate synthase (NADPH) |
molecular biology |
GltB1 shows 74% identity to the corresponding protein of Oceanobacillus iheyensis |
| 1.4.1.13 | glutamate synthase (NADPH) |
molecular biology |
GltB2 shows 73% identity to the glutamate synthase of Geobacillus kaustophilus |
| 1.4.1.13 | glutamate synthase (NADPH) |
molecular biology |
sequence identity between Salmonella typhimurium and Escherichia coli GOGAT is 91%, Salmonella typhimurium GOGAT region can complement an Escherichia coli mutant defective in GOGAT |
| 1.4.1.2 | glutamate dehydrogenase |
molecular biology |
GDH is essential for the full development of the secretory response in beta-cells |
| 1.4.1.2 | glutamate dehydrogenase |
molecular biology |
GDH, in conjunction with NADH-glutamte synthase, contributes to the control of leaf glutamate homeostasis, an amino acid that plays a central signaling and metabolic role at the interface of the carbon and nitrogen assimilatory pathways |
| 1.4.1.4 | glutamate dehydrogenase (NADP+) |
molecular biology |
promoter of GDH from Xanthophyllomyces dendrorhous is shown to be a valuable tool for controlled gene expression in Basidiomycetes |
| 1.4.1.4 | glutamate dehydrogenase (NADP+) |
molecular biology |
results implicate glutamate dehydrogenase and NADP-GDH in particular, as a key target of in vivo isophthalate inhibition during ammonium assimilation |
| 1.4.3.1 | D-aspartate oxidase |
molecular biology |
This enzyme is proposed to have a role in the inactivation of the synaptically released D-aspartate. Its C-terminus has a peroxisome targeting signal. |
| 1.5.1.11 | D-octopine dehydrogenase |
molecular biology |
His-tag-induced crystallization of OcDH is found to be dependent on the length of the His tag |
| 1.8.1.15 | mycothione reductase |
molecular biology |
a DTNB-coupled assay is developed for time-dependent inhibition of Mycobacterium tubercolosis reductase employing a benzyl glycoside analogue of MSH, from which an efficient mixed disulfide substrate is chemically recycled in situ, thereby greatly reducing the substrate quantities needed for such assays |
| 1.8.3.1 | sulfite oxidase |
molecular biology |
molybdenum trioxide (MoO3) nanoparticles display an intrinsic biomimetic sulfite oxidase activity under physiological conditions, and, functionalized with a customized bifunctional ligand containing dopamine as anchor group and triphenylphosphonium ion as targeting agent, they selectively target the mitochondria while being highly dispersible in aqueous solutions. Chemically induced sulfite oxidase knockdown cells treated with MoO3 nanoparticles recover their sulfite oxidase activity in vitro, which makes MoO3 nanoparticles a potential therapeutic for sulfite oxidase deficiency and opens new avenues for cost-effective therapies for gene-induced deficiencies. Molybdenum trioxide (MoO3) is a well-known model compound for selective oxidation catalysis |
| 2.1.1.35 | tRNA (uracil54-C5)-methyltransferase |
molecular biology |
enzyme is used for detecting tRNA-like moieties in viral RNA |
| 2.1.1.5 | betaine-homocysteine S-methyltransferase |
molecular biology |
osmotic regulation of BHMT may be part of a cell volume-regulatory response and additionally lead to metabolic alterations that depend on the availability of betaine-derived methyl groups |
| 2.1.1.5 | betaine-homocysteine S-methyltransferase |
molecular biology |
S-adenosylmethionine and 5-methylthioadenosine down-regulate BHMT expression in HepG2 cells in part by inducing NF-kappaB, which acts as a repressor for the human BHMT gene. While S-adenosylmethionines mechanism is NF-kappaB-dependent, 5-methylthioadenosine has both NF-kappaB-dependent and -independent mechanisms |
| 2.1.1.5 | betaine-homocysteine S-methyltransferase |
molecular biology |
the BHMT/betaine system directly protects hepatocytes from homocysteine-induced injury but not tunicamycin-induced injury, including an endoplasmic reticulum stress response, lipid accumulation, and cell death |
| 2.1.1.63 | methylated-DNA-[protein]-cysteine S-methyltransferase |
molecular biology |
modest binding cooperativity and high binding densities of AGT are adaptations that allow the enzyme to efficiently search for lesions in the context of chromatin remodeling and DNA replication |
| 2.1.1.77 | protein-L-isoaspartate(D-aspartate) O-methyltransferase |
molecular biology |
betaine administration of rats prevents the ethanol-induced accumulation of isoaspartyl-containing proteins in the liver by restoring the PIMT-catalyzed protein repair reaction through normalizing the hepatocellular SAM:SAH ratios |
| 2.1.2.5 | glutamate formimidoyltransferase |
molecular biology |
bifunctional formiminotransferase cyclodeaminase provides a novel marker to study ER-Golgi dynamics |
| 2.2.1.2 | transaldolase |
molecular biology |
TAL deficiency is shown as a modulator of mitochondrial homoeostasis, Ca2+ fluxing and apoptosis |
| 2.2.1.6 | acetolactate synthase |
molecular biology |
the G95A mutation of the ALS gene confers highly specific resistance to pyrimidinyl carboxy herbicides and can be used as a selection marker for transformations |
| 2.2.1.6 | acetolactate synthase |
molecular biology |
the gene is useful as a selectable marker for introducing foreign traits into rice when used with pyrimidinylcarboxylate herbicides. The double-mutant W548L/S627I of the ALS gene from rice is not only helpful for introducing useful rice genes into rice by self-cloning as a host-derived selectable marker gene but also can extinguish the scientific concern for antibiotic-resistant genes, leading to minimize public concern for this issue in transgenic plants |
| 2.3.1.12 | dihydrolipoyllysine-residue acetyltransferase |
molecular biology |
dihydrolipoamide acetyltransferase is shown to be a metabolic longevity factor and is required for calorie restriction-mediated life span extension |
| 2.3.1.158 | phospholipid:diacylglycerol acyltransferase |
molecular biology |
the conversion of a membrane bound lipid metabolizing enzyme into a soluble and active form might be applied to other enzymes with a membrane anchor region. |
| 2.3.1.183 | phosphinothricin acetyltransferase |
molecular biology |
the bar gene represents a selectable and assayable reporter gene especially suitable for 3-terminal gene fusions |
| 2.3.1.216 | 5,7-dihydroxy-2-methylchromone synthase |
molecular biology |
use of pentaketide chromone synthase for rational biosynthetic engineering to generate molecular diversity and pursue innovative, biologically potent compounds |
| 2.3.1.28 | chloramphenicol O-acetyltransferase |
molecular biology |
the chloramphenicol acetyl transferase gene serves as integration target for the eukaryotic mariner transposon Mos1 regardless of the location (chromosome or plasmid), as tested with in vitro and bacterial transposition assays |
| 2.3.1.28 | chloramphenicol O-acetyltransferase |
molecular biology |
single-chain variable fragment (scFv) phages are selected with affinity for CAT. Surface plasmon resonance analyses shows that the tested scFv phages have an affinity for CAT with a dissociation constant (Kd) around 1 microM. The selected scFv phages can be used as capture elements in a highly sensitive sandwich ELISA to detect CAT concentration as low as 0.1 ng/ml or 4 pM |
| 2.3.1.28 | chloramphenicol O-acetyltransferase |
molecular biology |
the enzyme mutant CATA138T may be useful as a genetic marker in Geobacillus spp. |
| 2.3.1.30 | serine O-acetyltransferase |
molecular biology |
results show that mitochondria provide the bulk of OAS in the plant cell and are the likely site of flux regulation |
| 2.3.1.30 | serine O-acetyltransferase |
molecular biology |
results show that some transgenic plants expressing serine acetyltransferase and cysteine synthase can mitigate detrimental effects of cadmium toxicity, perhaps by efficiently producing and accumulating sulfuric compounds |
| 2.3.1.31 | homoserine O-acetyltransferase |
molecular biology |
the HOA gene can be used as a selectable marker for transformation of Gibberella zeae |
| 2.3.1.4 | glucosamine-phosphate N-acetyltransferase |
molecular biology |
a microtiter plate based assay to detect the GlmU activity is developed: the assay relies on the enzymes MurA and MurB to convert UDP-GlcNAc into UDP-MurNAc with the concomitant reduction of NADPH. When all enzymes and substrates are present NADPH is oxidized with a concomitant decrease in OD340 |
| 2.3.1.4 | glucosamine-phosphate N-acetyltransferase |
molecular biology |
an assay for glucosamine-6-phosphate synthase using a yeast glucosamine-6-phosphate N-acetyltransferase 1 (GNA1) as coupling enzyme is developed. The assay measures the production of glucosamine-6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,50-dithio-bis(2-nitrobenzoic acid) (Ellmans reagent) in a discontinuous manner. This method is simple to perform and can be adapted to a 96-well microtiter plate format |
| 2.3.1.48 | histone acetyltransferase |
molecular biology |
the catalytically inactive mutant Y891F is useful for stable binding and purification of unacetylated histone H4 protein |
| 2.3.1.48 | histone acetyltransferase |
molecular biology |
the results demonstrate the importance of TIPs in the recruitment of p300 to specific promoters and in the regulation of p300 HAT activity through the involvement of the SANT domain |
| 2.3.1.5 | arylamine N-acetyltransferase |
molecular biology |
results demonstrate that human P4501A1 and NATs (NAT1 and NAT2) contribute significantly to the activation of PBTA-type compounds to genotoxic metabolites that induce umuC gene expression in Salmonella typhimurium tester strains |
| 2.3.1.5 | arylamine N-acetyltransferase |
molecular biology |
results show that cisplatin inactivates the NAT1 enzyme by forming an adduct with the catalytic cysteine residue of the enzyme |
| 2.3.1.50 | serine C-palmitoyltransferase |
molecular biology |
results show that SPT modulated programmed cell death plays an important role in the regulation of male gametophyte development of Arabidopsis thaliana |
| 2.3.1.97 | glycylpeptide N-tetradecanoyltransferase |
molecular biology |
the enzyme can be used for protein N-myristoylation as a tag labeling technique in recombinant expresssion systems. CaNMT is an effective tool for in vitro and in vivo transfer of an azide-modified acid to the N-terminus of a polypeptide derived from a species entirely unrelated to Candida albicans |
| 2.3.1.B25 | octaketide synthase |
molecular biology |
use of octaketide synthase for rational biosynthetic engineering to generate molecular diversity and pursue innovative, biologically potent compounds |
| 2.4.1.101 | alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase |
molecular biology |
production of rare hybrid oligosaccharides for biochemical and structural studies, 100% conversion of oligosaccharide substrate at room temperature, yield of 42% after purification from reaction mixture |
