EC Number   |
Application |
Reference |
|---|
   1.2.1.75 | biotechnology |
the crystallographic data indicate how to construct a bispecific cofactor binding site and to engineer a malonyl-CoA into methylmalonyl-CoA reductase for polyester building block production |
-, 722819 |
| 1.2.1.75 | synthesis |
developments in 3-hydroxypropionate (HP) production involving the enzyme using Saccharomyces cerevisiae as an industrial host. By combining genome-scale engineering tools, malonyl-CoA biosensors and optimization of downstream fermentation, the production of 3-HP in yeast has the potential to reach or even exceed the yield of chemical production |
763082 |
| 1.2.1.75 | synthesis |
integration of multiple copies of malonyl-CoA reductase MCR and of phosphorylation-deficient acetyl-CoA carboxylase ACC1 genes into the genome of yeast increases 3-hydroxypropionic acid titer fivefold in comparison with single integration. Optimizing the supply of acetyl-CoA by overexpressing native pyruvate decarboxylase PDC1, aldehyde dehydrogenase ALD6, and acetyl-CoA synthase from Salmonella enterica SEacsL641P engineering the cofactor specificity of the glyceraldehyde-3-phosphate dehydrogenase to increase the intracellular production of NADPH at the expense of NADH improves 3-hydroxypropionic acid production and reduces formation of glycerol as by-product. The final strain produces 9.8 g per L 3-hydroxypropionic acid with a yield of 13% C-mol per C-mol glucose after 100 h in carbon-limited fed-batch cultivation at pH 5 |
740966 |
| 1.2.1.75 | synthesis |
MCR is of biotechnological interest for the synthesis of polyester building blocks |
-, 722819 |
| 1.2.1.75 | synthesis |
the combination of Escherichia coli BL21(DE3) and pET28a carrying heterogeneous acetyl-CoA carboxylase (Acc) from Corynebacterium glutamicum and codon-optimized malonyl-CoA reductase (MCR) from Chloroflexus aurantiacus is the most efficient host-vector system for 3-hydroxypropionic acid production, and the highest concentration of 3-hydroxypropionic attained in shake flask cultivation reaches 1.80 g/l with induction at 0.25 mM IPTG and 25°C, and supplementation of NaHCO3 and biotin |
740172 |
| 1.2.1.75 | synthesis |
the enzyme is very useful for 3-hydroxypropionate biosynthesis and production |
762940 |
