EC Number   |
Application |
Reference |
|---|
    1.3.99.4 | molecular biology |
development of a synthetic 3-ketosteroid DELTA1-dehydrogenase for the generation of a catabolic pathway enabling cholesterol degradation in human cells |
763735 |
| 1.3.99.4 | more |
3-oxosteroid DELAT1-dehydrogenases are of particular interest for the etiology of some infectious diseases, for the production of starting materials for the pharmaceutical industry, and for environmental bioremediation applications |
763443 |
| 1.3.99.4 | more |
ksdD-1 and ksdD-2 display respectively high (78%) and low (33%) amino acid sequence identity with the putative ksdD gene of Mycobacterium tuberculosis |
-, 675829 |
| 1.3.99.4 | more |
the two putative Mycobacterium tuberculosis KsdDs, MT3641 and MT0809, complement the Mycobacterium smegmatis deltaksdD-1 deltaksdD-2 double mutant |
-, 675829 |
| 1.3.99.4 | synthesis |
engineered enzyme mutants, in which KsdDM is inactivated or augmented, are useful for production of phytosterols 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione, circumventing the difficulty of separating 4-androstene-3,17-dione from 1,4-androstadiene-3,17-dione, a key bottleneck to the microbial transformation of phytosterols in industry |
-, 724000 |
| 1.3.99.4 | synthesis |
inactivation of KstD isoforms 1-3 leads to synthesis of about 6.02g/l of 9alpha-hydroxy-4-androstene-3,17-dione from 15 g/l of phytosterols. The product is mixed with 1.55 g/l of 4-androstene-3,17-dione as a major byproduct. Overexpression of the oxygenase component of 3-ketosteroid-9alpha-hydroxylase kshA, results in a yield of 9alpha-hydroxy-4-androstene-3,17-dione of 7.33 g/l |
-, 743221 |
| 1.3.99.4 | synthesis |
kstDF is a promising enzyme in steroid DELTA1-dehydrogenation and steroid reduction that is propitious to construct genetically engineered steroid-transforming recombinants by heterologous overexpression |
-, 724074 |
| 1.3.99.4 | synthesis |
recombinant enzyme application potential of the former in the synthesis of prednisolone, method evaluation and optimization |
-, 762654 |
