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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 1.4.3.5Display the word mapDisplay the reaction diagram Show all sequences 1.4.3.5- 391873, 391875, 391877, 391890, 391891, 391895, 391901, 391905, 657296, 765052, 765076
Show all pathways known for 1.4.3.5Display the word mapDisplay the reaction diagram Show all sequences 1.4.3.5recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, removal of the GST-tag by rTEV protease, followed by dialysis and ultrafiltration 667048
Show all pathways known for 1.4.3.5Display the word mapDisplay the reaction diagram Show all sequences 1.4.3.5native enzyme with tightly bound FMN 667050
Show all pathways known for 1.4.3.5Display the word mapDisplay the reaction diagram Show all sequences 1.4.3.5recombinant GST-tagged wild-type and of GST-tagged selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, removal of the GST-tags by rTEV protease, followed by dialysis and ultrafiltration 667083
Show all pathways known for 1.4.3.5Display the word mapDisplay the reaction diagram Show all sequences 1.4.3.5recombinant enzyme from Escherichia coli strain BL21 (DE3) to homogeneity 712164
Show all pathways known for 1.4.3.5Display the word mapDisplay the reaction diagram Show all sequences 1.4.3.5Ni-NTA column chromatography 726286
Show all pathways known for 1.4.3.5Display the word mapDisplay the reaction diagram Show all sequences 1.4.3.5nickel affinity column chromatography 741499
Show all pathways known for 1.4.3.5Display the word mapDisplay the reaction diagram Show all sequences 1.4.3.5HisTrap nickel-affinity column chromatography and Superdex 200 gel filtration 742525
Show all pathways known for 1.4.3.5Display the word mapDisplay the reaction diagram Show all sequences 1.4.3.5His Trap column chromatography and Superdex 200 gel filtration 743584
Show all pathways known for 1.4.3.5Display the word mapDisplay the reaction diagram Show all sequences 1.4.3.5recombinantly expressed mutant enzyme R118Q 764548
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