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Search Purification (Commentary)

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<< < Results 11 - 17 of 17
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1recombinant domain I 392665, 392667
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1recombinant domain I and recombinant E155W and Y171W mutant domain III 392674
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1recombinant domains I and III 392665
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1recombinant protein 657468, 658003
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1recombinant proteins 657960
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1recombinant wild-type, T393C, R425C, G430C and A432C mutant domain III 392667
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1the recombinant enzyme is purified by pre-extraction of the membranes with sodium cholate and Triton X-100, solubilization of the enzyme with sodium deoxycholate in the presence of 1 M potassium chloride, and centrifugation through a 1.1 M sucrose solution. The wild type enzyme is purified by phenyl Sepharose column chromatography, DEAE-Bio-Gel A column chromatography, and NAD+ agarose column chromatography 392618
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